Log In Sign up
The largest database of trusted experimental protocols

Ht multitron standard

Manufactured by Infors
Visit Supplier
Sourced in Switzerland

The HT Multitron Standard is a high-performance benchtop incubator shaker designed for a wide range of cell culture and microbiology applications. It features precise temperature and shaking control to provide optimal growth conditions for a variety of cell lines and microorganisms.

Automatically generated - may contain errors

Lab products found in correlation

Evolution 60, by Thermo Fisher Scientific (1 mentions) Finnigan ltq, by Thermo Fisher Scientific (1 mentions) Vibra cell vcx130, by Sonics (1 mentions) Gen 3 microplate, by Biolog (1 mentions)

Close spelling variants of this product

HT Multitron Standard incubator Infors HT Multitron Multitron Standard Multitron

9 protocols using ht multitron standard

1

Humic Acid Adsorption Capacity of Synthesized Carbons

Check if the same lab product or an alternative is used in the 5 most similar protocols
The HA adsorption capacity
of the synthesized carbons was determined using the methodology described
by Libbrecht et al.(67 (link)) First,
a 500 mg/L HA solution was prepared by dispersing 500 mg of HA in
1 L of deionized water. HA was dissolved by adding 0.1 M sodium hydroxide
solution until it reached pH 12, and ultrasonic treatment was carried
out for 30 min. After dissolution, the solution was neutralized to
pH 7 with 0.1 M hydrochloric acid solution. Next, 10 mg of the adsorbent
was added to 50 mL of the 500 mg/L HA solution. These mixtures were
incubated in a thermostatic shaker (Infors HT Multitron Standard)
at 25 °C and 200 rpm for 7 days to ensure equilibrium. After
incubation, the solution was filtered through a 0.45 μm PET
syringe filter, and its HA concentration was measured by UV–vis
at 254 nm using a Thermo Scientific Evolution 60 UV–vis spectrometer
with VISIONlight 4 software. The HA concentration of the 500 mg/L
solution was determined by the same procedure (filtration and UV–vis
analysis). All adsorption experiments were performed in triplicate.
The adsorption capacity was calculated using the equation where qe (mg/g)
is the HA adsorption capacity at equilibrium, C0 and Ce (mg/L) are the initial
and equilibrium concentration of HA, m (mg) is the
mass of the adsorbent, and V (L) is the solution
volume.
Jedrzejczyk M.A., Engelhardt J., Djokic M.R., Bliznuk V., Van Geem K.M., Verberckmoes A., De Clercq J, & Bernaerts K.V. (2021). Development of Lignin-Based Mesoporous Carbons for the Adsorption of Humic Acid. ACS Omega, 6(23), 15222-15235.
+ Open protocol
+ Expand
2

Growth Kinetics of Hfx. mediterranei R-4

Check if the same lab product or an alternative is used in the 5 most similar protocols
Hfx. mediterranei strain R-4 (ATCC33500) (isolated from saltern ponds located in Santa Pola, Alicante, Spain) was used for all experiments. Cells were grown in a complex medium containing 25% (w/v) of inorganic salts [27 (link)] and 0.5% (w/v) yeast extract. The pH was adjusted to 7.3 using NaOH and HCl solutions. Growth conditions included 42 °C and shaking at 170 rpm (Infors HT Multitron Standard). Growth specific velocity (µ) (1) and duplication time (Dt) (2) were calculated for each condition using the following equations:

Giani M, & Martínez-Espinosa R.M. (2020). Carotenoids as a Protection Mechanism against Oxidative Stress in Haloferax mediterranei. Antioxidants, 9(11), 1060.
+ Open protocol
+ Expand
3

Culturing Streptomyces ambofaciens ATCC 23877

Check if the same lab product or an alternative is used in the 5 most similar protocols
S. ambofaciens ATCC 23877 was grown on solid soy flour-mannitol (SFM) medium 59 at 28°C unless otherwise indicated. The strain was grown in the following media: MP5 medium (7 g/l yeast extract, 20.9 g/l MOPS, 5 g/l NaCl, 1 g/l NaNO3, 36 ml/l glycerol -pH 7.5) 34 (link) , or YEME medium 10.3 % sucrose (3 g/l yeast extract, 5 g/l bactotryptone, 3 g/l malt extract, 10 g/l glucose, 103 g/l sucrose; pH 7.0-7.2 ; adapted from 59 ). Twenty million spores of S. ambofaciens ATCC 23877 were inoculated in 100 ml of media before growth at 30°C in a shaking agitator (220 rpm, INFORS HT multitron standard).
Lioy V.S., Lorenzi J., Najah S., Poinsignon T., Leh H., Saulnier C., Aigle B., Lautru S., Thibessard A., Lespinet O., Leblond P., Jaszczyszyn Y., Gorrichon K., Varoquaux N., Junier I., Boccard F., Pernodet J., & Bury‐Moné S. (2020). Dynamics of the compartmentalizedStreptomyceschromosome during metabolic differentiation.
+ Open protocol
+ Expand
4

Culturing Streptomyces ambofaciens ATCC 23877

Check if the same lab product or an alternative is used in the 5 most similar protocols
S. ambofaciens ATCC 23877 was grown on solid soy flour-mannitol (SFM) medium 59 at 28°C unless otherwise indicated. The strain was grown in the following media: MP5 medium (7 g/l yeast extract, 20.9 g/l MOPS, 5 g/l NaCl, 1 g/l NaNO3, 36 ml/l glycerol -pH 7.5) 34 (link) , or YEME medium 10.3 % sucrose (3 g/l yeast extract, 5 g/l bactotryptone, 3 g/l malt extract, 10 g/l glucose, 103 g/l sucrose; pH 7.0-7.2 ; adapted from 59 ). Twenty million spores of S. ambofaciens ATCC 23877 were inoculated in 100 ml of media before growth at 30°C in a shaking agitator (220 rpm, INFORS HT multitron standard).
Lioy V.S., Lorenzi J., Najah S., Poinsignon T., Leh H., Saulnier C., Aigle B., Lautru S., Thibessard A., Lespinet O., Leblond P., Jaszczyszyn Y., Gorrichon K., Varoquaux N., Junier I., Boccard F., Pernodet J., & Bury‐Moné S. (2021). Dynamics of the compartmentalized Streptomyces chromosome during metabolic differentiation.
+ Open protocol
+ Expand
5

Ultrasonic Liquid Processing and Analytical Workflows

Check if the same lab product or an alternative is used in the 5 most similar protocols
A Vibra-cell VCX130 ultrasonic liquid processor (Sonics), an Äkta 900 equipped with a fraction collector (GE Healthcare), and Waters UPLC and HPLC systems were used. For ESI-MS, a Finnigan LTQ (Thermo Fisher Scientific) was employed. A lyophilizer instrument (FreeZone 2.5 Plus) and a shaking incubator with a temperature regulator (Infors HT multitron Standard) were also used in the experiments.
Chiki A., Ricci J., Hegde R.N., Abriata L.A., Reif A., & Lashuel H.A. (2020). Site-specific phosphorylation of Huntingtin exon 1 recombinant proteins enabled by the discovery of novel kinases.
+ Open protocol
+ Expand
6

Phenotypic Characterization of Microbes

Check if the same lab product or an alternative is used in the 5 most similar protocols
The GEN III MicroPlates TM (Biolog, Inc.) system was used for phenotypic characterization of carbon and nitrogen sources utilization and sensitivity to some chemicals, following the manufacturer's instructions except for the following modifications. Strains were grown on TY plates, cultures were resuspended in IF-A inoculation fluid (GEN III MicroPlates TM , Biolog, Inc.) and adjusted to an O.D 600 nm of 0.2. Portions of 0.1 ml were added to each well, and plates were incubated at 28ºC at 150 rpm on a rotary shaking (Infors HT Multitron standard) for 48 h. For growth at different pH, Yeast Mannitol (YM) medium was adjusted to pH 8-9.6 with 50 mM Tris.
Soenens A., Gomila M, & Imperial J. (2019). Neorhizobium tomejilense sp. nov., first non-symbiotic Neorhizobium species isolated from a dryland agricultural soil in southern Spain. Systematic and applied microbiology, 42(2).
+ Open protocol
+ Expand
7

Bacterial Preculture Preparation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
A single colony from an agar plate, previously incubated for 3–4 days at 30°C, was used to inoculate the first preculture in 10 ml TSB media in a 125‐mL Ultra Yield flask (Thomson Instrument Company), sealed with an AirOtop membrane (Thomson Instrument Company). At an OD600nm ≥5 after 13–15 h of incubation, 3 ml were transferred to a second preculture containing 300 ml MSM. The second preculture was incubated for 24 h in a 1000‐ml DURAN baffled glass flask (DWK Life Sciences GmbH) sealed with an AirOtop membrane. An orbital shaker (INFORS HT Multitron Standard, Infors AG) was used for the incubation of both precultures at 30°C, 200 rpm and with a shaking amplitude of 25 mm.
Gutschmann B., Högl T.H., Huang B., Maldonado Simões M., Junne S., Neubauer P., Grimm T, & Riedel S.L. (2022). Polyhydroxyalkanoate production from animal by‐products: Development of a pneumatic feeding system for solid fat/protein‐emulsions. Microbial Biotechnology, 16(2), 286-294.
+ Open protocol
+ Expand
8

Blood-Plasma Partitioning of Actinomycin D

Check if the same lab product or an alternative is used in the 5 most similar protocols
An extraction solution of 1:1 deionized water and acetonitrile with 0.1% zinc sulphate was prepared on the day of the experiment and Act D solutions of 4, 20 and 100 μg ml−1 were prepared in saline. All experiments were performed in triplicate. An aliquot of 180 μl whole blood from a healthy donor was added to 20 μL of each Act D solution (final concentrations 0.4, 2 and 10 μg ml−1) in Eppendorf tubes, followed by gentle rotation for 1 min before transfer to an incubator (Infors HT Multitron Standard, Infors AG, Basel) at 37 °C for 1 h and 40 μl of plasma was transferred to a new Eppendorf and mixed with 40 μl of fresh blood.
For the blood samples, 40 μl of blood was transferred to a new Eppendorf and mixed with 40 μl blank plasma. All samples were then mixed with 160 μl of the extraction solution and frozen at −80 °C for 1 h. Samples were then centrifuged at 2000 g and 4 °C for 1 h and 100 μl of supernatant from each sample was transferred to HPLC vials and stored at −20 °C overnight. LC–MS analysis was performed as described above for the analysis of transporter kinetic samples. The blood‐to‐plasma ratio was calculated from the following equation (where Kb/p is the whole blood‐to‐plasma partition coefficient, Ke/p is the red blood cell‐to‐plasma partition and H is the haematocrit):
Blood‐to‐plasmaKb/p=Ke/p×H+1−H
Walsh C., Bonner J.J., Johnson T.N., Neuhoff S., Ghazaly E.A., Gribben J.G., Boddy A.V, & Veal G.J. (2016). Development of a physiologically based pharmacokinetic model of actinomycin D in children with cancer. British Journal of Clinical Pharmacology, 81(5), 989-998.
+ Open protocol
+ Expand
9

Cultivation of Ralstonia eutropha for PHA Production

Check if the same lab product or an alternative is used in the 5 most similar protocols
R. eutropha Re2058/pCB113 was streaked on a TSB agar plate from a cryoculture and incubated for 3–4 days at 30 °C. The first preculture in 10 mL TSB media in a 125-mL Ultra Yield flask (Thomson Instrument Company, USA), sealed with an AirOtop membrane (Thomson Instrument Company, USA), was inoculated with a single colony from the plate. After 14 h of incubation an optical density (OD600) of 4–5 was reached and 0.5 mL of the culture were used to inoculate the second preculture (50 mL MSM containing 1 wt% canola oil) in a 250-mL DURAN baffled glass flask (DWK Life Sciences GmbH, Germany) sealed with an AirOtop membrane. After 24 h of incubation, 0.5 mL of the seed culture were inoculated into a 250-mL DURAN baffled glass flask with 50 mL mineral salt media containing 1–2 wt% of the animal by-product. Each condition was tested in triplicates and all cultures were incubated at 30 °C and shaken at 200 rpm in an orbital shaker with 25 mm amplitude (INFORS HT Multitron Standard, Infors AG, Switzerland).
Saad V., Gutschmann B., Grimm T., Widmer T., Neubauer P, & Riedel S.L. (2020). Low-quality animal by-product streams for the production of PHA-biopolymers: fats, fat/protein-emulsions and materials with high ash content as low-cost feedstocks. Biotechnology Letters, 43(3), 579-587.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!