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Gaussia juice kit

Manufactured by Promega
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The Gaussia-Juice Kit is a product designed to detect and quantify the Gaussia luciferase reporter gene. The kit provides the necessary components to measure Gaussia luciferase activity in cell culture, tissue, or other biological samples.

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Lab products found in correlation

Orion microplate luminometer, by Berthold Technologies (2 mentions) Luciferase assay, by Promega (1 mentions) Aluciferase assay kit, by Promega (1 mentions)

2 protocols using gaussia juice kit

1

NF-κB Activation Measurement in HEK293T Cells

Cited 2 times
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HEK293T cells were cotransfected with a firefly luciferase reporter construct under the control of three NF-κB binding sites55 (link), a Gaussia luciferase construct55 (link) for normalization, and expression vectors for a constitutively active mutant of IKKβ56 (link) and the functional vpu alleles as described15 (link). Two days post-transfection, Gaussia luciferase activity in the supernatant was measured using the Gaussia-Juice Kit (p.j.k.) and the cells were used to measure firefly luciferase activity using the Luciferase Assay from Promega with an Orion Microplate luminometer (Berthold).
Joas S., Parrish E.H., Gnanadurai C.W., Lump E., Stürzel C.M., Parrish N.F., Learn G.H., Sauermann U., Neumann B., Rensing K.M., Fuchs D., Billingsley J.M., Bosinger S.E., Silvestri G., Apetrei C., Huot N., Garcia-Tellez T., Müller-Trutwin M., Hotter D., Sauter D., Stahl-Hennig C., Hahn B.H, & Kirchhoff F. (2018). Species-specific host factors rather than virus-intrinsic virulence determine primate lentiviral pathogenicity. Nature Communications, 9, 1371.
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2

IFNβ Promoter Activity Assay

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To determine the effect of IFI16 on IFNβ-promoter activity,
22,000 HEK293T cells/well were seeded in poly-L-lysine-coated 96-well plates
and transfected in triplicates using the calcium-phosphate transfection
method. Cells were cotransfected with a firefly luciferase reporter
construct under the control of the IFNβ-promoter (100 ng), a
Gaussia luciferase construct under the control of a
constitutively active pTAL promoter (5 ng) for normalization, and expression
vectors or proviral constructs (50 ng). As a positive control, the
IFNβ-promoter was activated by infection of the cells with Sendai
virus for 24 h. 40 h after transfection, activity of the secreted
Gaussia luciferase was determined in the cell culture
supernatants using the Gaussia- Juice Kit (p.j.k.), whereas
firefly luciferase activity was determined in cell lysates using a
Luciferase Assay Kit (Promega) according to the manufacturer’s
instructions with an Orion microplate luminometer (Berthold). Firefly
luciferase signals were normalized to the corresponding
Gaussia luciferase control values to normalize for
transfection efficiency.
Hotter D., Bosso M., Jønsson K.L., Krapp C., Stürzel C.M., Das A., Littwitz-Salomon E., Berkhout B., Russ A., Wittmann S., Gramberg T., Zheng Y., Martins L.J., Planelles V., Jakobsen M.R., Hahn B.H., Dittmer U., Sauter D, & Kirchhoff F. (2019). IFI16 targets the transcription factor Sp1 to suppress HIV-1 transcription and latency reactivation. Cell host & microbe, 25(6), 858-872.e13.
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