Cd3 pe cyanine7

Manufactured by BioLegend

The CD3-PE-Cyanine7 is a fluorescent-labeled antibody that binds to the CD3 antigen, a key component of the T cell receptor complex. It is commonly used in flow cytometry applications to identify and quantify T cells in biological samples.

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Lab products found in correlation

Cd4 fitc, by BioLegend (2 mentions) Cd62l apc, by BD (1 mentions) Cd44 pe, by BioLegend (1 mentions) Facscalibur flow cytometer, by BD (1 mentions) Foxp3 pe, by Thermo Fisher Scientific (1 mentions) Cd8 pe, by BioLegend (1 mentions) Igg1 fitc, by Thermo Fisher Scientific (1 mentions) Igg1 pe, by Thermo Fisher Scientific (1 mentions) Fixation permeabilization solution kit, by Thermo Fisher Scientific (1 mentions) Cd25 apc, by BioLegend (1 mentions) Collagenase type 4, by STEMCELL (1 mentions) Cd8 fitc, by BioLegend (1 mentions) Granzyme b apc, by BioLegend (1 mentions)

Spelling variants of this product

CD3-PE (53 citations) PE/Cyanine7 (12 citations) PE/Cyanine7 anti-human CD3 (5 citations) PE/Cyanine7-labeled anti-mouse CD3 (2 citations)

3 protocols using cd3 pe cyanine7

Quantifying SARS-CoV-2 Specific T Cell Responses

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T cell proliferation in immunized mice were evaluated using a FACSCalibur flow cytometer (BD Biosciences). Briefly, a total of 1,000,000 mouse splenocytes were stimulated with SARS-CoV-2 RBD peptide pool (2 μg/ml of each peptide, see Table S3) for 2 hours at 37°C with 5% CO₂. Brefeldin A (1 μg/ml, MCE) was then added into splenocytes and incubated for 4 hours. Following two washes with PBS, splenocytes were permeabilized and stained with fluorescently conjugated antibodies to CD3 (PE/Cyanine7) (BioLegend), CD4 (FITC) (BioLegend), CD8 (APC/FITC) (BioLegend), CD44 (PE) (BioLegend) or CD62L (APC) (BD Biosciences). Dead cells were stained with Zombie UV3 fixable viability Kit (BioLegend). Data are analyzed with FlowJo software.

Zhang N.N., Li X.F., Deng Y.Q., Zhao H., Huang Y.J., Yang G., Huang W.J., Gao P., Zhou C., Zhang R.R., Guo Y., Sun S.H., Fan H., Zu S.L., Chen Q., He Q., Cao T.S., Huang X.Y., Qiu H.Y., Nie J.H., Jiang Y., Yan H.Y., Ye Q., Zhong X., Xue X.L., Zha Z.Y., Zhou D., Yang X., Wang Y.C., Ying B, & Qin C.F. (2020). A Thermostable mRNA Vaccine against COVID-19. Cell, 182(5), 1271-1283.e16.

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Flow Cytometry Analysis of Immune Cells

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Mouse anti-human CD3-PE-Cyanine7 (BioLegend, Clone: UCHT1), CD4-FITC (BioLegend, Clone: RPA-T4), CD8-PE (BioLegend, Clone: RPA-T8), CD25-APC (BioLegend, Clone: BC96), Foxp3-PE (eBioscience, Clone: 236A/E7), IgG1-PE (eBioscience, Clone: P3.6.2.8.1), IgG1-FITC (eBioscience, Clone: P3.6.2.8.1), IgG2b-PE-Cyanine7 (BD Pharmingen, Clone: A95-1) and IgG2a-APC (eBiocience, Clone: eBR2a) antibodies were used for flow cytometry analysis. Patients’ blood samples as well as peripheral blood and single-cell suspension of target organs of mice at the time of necropsy were analyzed by flow cytometry. Briefly, red blood cells were treated with Lysing Buffer (BD Pharm LyseTM) at 4°C for 15 min according to the protocol. Intracellular Foxp3 staining was performed according to the protocol (Fixation/Permeabilization Solution Kit; eBioscience). All samples were incubated with antibodies or isotype matched control IgG for 30 min in the dark. Then, flow cytometric analyses were performed on the Flow Cytometer (BD FACS Canto II) and further analyzed using FlowJo 10.0.

Bu X., Pan W., Wang J., Liu L., Yin Z., Jin H., Liu Q., Zheng L., Sun H., Gao Y, & Ping B. (2023). Therapeutic Effects of HLA-G5 Overexpressing hAMSCs on aGVHD After Allo-HSCT: Involving in the Gut Microbiota at the Intestinal Barrier. Journal of Inflammation Research, 16, 3669-3685.

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Analyzing Mouse Mammary Tumor Immune Cells

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Mouse mammary tumor nodes were excised and divided into two parts for histopathological analysis and flow cytometry analysis, respectively. Tumor tissues for flow cytometry were cut into small pieces and digested with 1 mg/mL collagenase type IV (07912; Stemcell) and 0.5 Kunits/ml DNase (D7073; Biotopped) for 0.5 h. Samples were then filtrated to single-cell suspension. Cells were stained with CD45 À PerCP-Cyanine5.5 (103132; Biolegend; 1:20), CD8-FITC (100706; Biolegend; 1:20), CD3-PE-Cyanine7 (100320; Biolegend; 1:20) and Granzyme B-APC (100706; Biolegend; 1:20). Subsequently, cells were analyzed by flow cytometry. The results were analyzed by FlowJo X.

Liu Y., Peng Y., Du W., Yu C., Peng Z., Qin L., Ma Y., Wu X., Peng Y., Cheng X., Xia L., Fa H., Wu Y., Sun L., Liu J., Liu Z., Shang Y., Wang S, & Liang J. (2023). PD-L1-mediated immune evasion in triple-negative breast cancer is linked to the loss of ZNF652. Cell reports, 42(11).

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