Cells and tissues were collected and lysated using RIPA buffer (100:1:1 ratio of phosphatase inhibitor to protease inhibitor). After 30 min of lysis on ice, the total protein was extracted. After separation by SDS-PAGE (AR0138, Boster Biotechnology), the protein was transferred onto a nitrocellulose membrane (AR0135-04, Boster Biotechnology). The cells and tissues were then blocked with 5% skim milk at room temperature for 2 h and incubated with an appropriate concentration of primary antibody overnight at 4 °C. Finally, the membranes were incubated with peroxidase-conjugated goat anti-rabbit IgG (H + L) antibody (abs20002ss; Absin Bioscience) at room temperature for 2 h. The immunoreactive bands were detected using western blotting.
Ar0135 04
Manufactured by Boster Bio
The AR0135-04 is a laboratory equipment product. It is designed for use in scientific research and analysis. The core function of this product is to perform specific tasks related to the lab environment. No further details on the intended use or functionality of this product can be provided in an unbiased and factual manner.
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Lab products found in correlation
2 protocols using ar0135 04
1
Protein Expression Analysis by Western Blot
2
Western Blot Protein Analysis
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Total protein from tissues or cells was lysed in RIPA buffer containing complete protease and phosphatase inhibitors. After separation by 8% or 10% SDS-PAGE (AR0138, Boster Biotechnology) and transfer to nitrocellulose membranes (AR0135-04, Boster Biotechnology), the membranes were blocked with 5% skimmed milk for 2 h at room temperature, and then incubated with the corresponding primary antibodies overnight at 4°C. Then membranes were incubated with HRP-conjugated goat anti-rabbit IgG (H + L) antibody (abs20002ss, Absin Bioscience, Shanghai, China) at room temperature for 2 h. Immunoreactive bands were detected using a western blot detection kit.
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