The mealworm extract was characterized in an Agilent 7890A GC-MS (Agilent Technologies, Santa Clara, CA, USA) as described by Navarro del Hierro et al. [16 (link)], and the previous derivatization of the samples by silylation with BSTFA at 10 mg/mL (60 min, 75 °C). The equipment comprised a split/splitless injector, G4513A autoinjector, an electronic pressure control and a 5975C triple-axis mass spectrometer detector. The column employed was an Agilent HP-5MS capillary column (30 m × 0.25 mm i.d., 0.25 µm-phase thickness) and the carrier gas was helium at a flow of 2 mL/min. Sample injections (1 µL) were performed in splitless mode. The injector temperature was 260 °C and the mass spectrometer ion source and interface temperatures were 230 and 280 °C, respectively. The temperature of the oven was initiated at 50 °C, held for 3 min and increased at a rate of 15 °C/min to 310 °C, which was then held for 25 min. The mass spectra were obtained by electronic impact at 70 eV. The scanning speed was 1.6 scans/s in a mass range of 30–700 amu. Identification of compounds was performed by the NIST MS data library, by the mass spectra according to literature, or according to commercial standards (most fatty acids, glycerides, sugars and sterols) previously derivatized following the same procedure as samples.
Agilent 7890a gc ms
Manufactured by Agilent Technologies
Sourced in United States
The Agilent 7890A GC-MS is a gas chromatography-mass spectrometry (GC-MS) system. It is designed to separate, identify, and quantify a wide range of chemical compounds. The 7890A GC-MS combines a gas chromatograph with a mass spectrometer to provide highly sensitive and accurate analysis of complex samples.
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4 protocols using agilent 7890a gc ms
1
GC-MS Analysis of Mealworm Extract
2
Policosanol Components Analysis
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The policosanol components were characterized and examined by an Agilent 7890A GC-MS (Agilent Technologies Inc., PaloAlto, CA, USA). The absorbance results were analyzed using a microplate plate reader (96 well, Molecular Devices, Sunnyvale, CA, USA). Lysate were transferred to nitrocellulose membranes by electrophoretic transfer cell (Bio-Rad, Hercules, CA, USA).
3
Spectral Analysis of Chemical Fractions
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Ultra violet-visible (UV-Vis) of fractions were taken on Du 730 Life Science UV/Vis spectrophotometer Beckman Coulter. Infrared (IR) spectra were recorded on SHIMADZU 8400s FTIR (Fourier Transform) spectrophotometer and gas chromatography-mass spectrometry was carried out with Agilent 7890A GC/MS equipped with a quadrupole mass spectra detector and an auto sampler. The GC-MS system settings are as follows: 2000 °C, interfaced temperature, 2500 °C, solvent cut time; 2.50 min; relative detector mode, ACQ mode; Scan; start time-end time, 3.00-56.00 min; event time, 0.50 s; scan speed 1428. Then, the GC system was equilibrated to a stable temperature of 2500 °C, the isolates were diluted with acetone and put into micro sample vials.
4
GC-MS Characterization of Derivatized Extracts
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The composition of the extracts were characterized by GC-MS after derivatization of the samples with BSTFA according to Herrera et al. (2019b) (link) with small modifications. Extracts were dissolved in BSTFA at a concentration of 20 mg/mL and heated at 75°C for 1 h. After, samples rested at room temperature for 5 minutes and then were centrifuged for 5 minutes at 4500 rpm (Centrifuge MiniSpin® plus). The supernatant was analyzed in an Agilent 7890A GC-MS (Agilent Technologies, Santa Clara, CA, USA). The column employed was an Agilent HP-5MS UI capillary column (30 m × 0.250 mm × 0.25 μm) and the carrier gas was helium with a flow of 2 mL/minute. A G4513A autoinjector was used, with 1 μL injections in splitless mode and the injector temperature was 260°C. The oven was initially set at 50 ºC and increased at 10 ºC/minute to 310 ºC, held for 25 minutes. The inlet temperatures at the MS were set at 260 °C and those at the MS ion source and the interface were 230 °C and 280 °C, respectively. The scanning speed was 0.79 scans/s in a mass range of 30-1000 amu. Identification of compounds was performed by the NIST MS Data library, by the mass spectra according to literature, or according to commercial standards (most fatty acids, glycerides, sugars, and sterols), previously derivatized following the same procedure as samples.
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