Anti batf sc 100974

Manufactured by Santa Cruz Biotechnology

Anti-BATF (sc-100974) is an antibody produced by Santa Cruz Biotechnology. It is designed to detect BATF (basic leucine zipper ATF-like transcription factor) in cellular and molecular biology applications.

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Lab products found in correlation

Ne per nuclear and cytoplasmic extraction reagent, by Thermo Fisher Scientific (2 mentions) Anti flag m2 conjugated agarose, by Merck Group (1 mentions) Anti jund sc 74, by Santa Cruz Biotechnology (1 mentions) Anti irf4 sc 6059, by Santa Cruz Biotechnology (1 mentions) Anti flag m2, by Merck Group (1 mentions) Anti ha 16b12, by Fortrea (1 mentions) Anti β actin sc 47778, by Santa Cruz Biotechnology (1 mentions) Ab23980, by Abcam (1 mentions) Ab20346, by Abcam (1 mentions) Ab18465, by Abcam (1 mentions) Ab12169, by Abcam (1 mentions) Ab17721, by Abcam (1 mentions) Anti hdac2, by Abcam (1 mentions) Anti chd4, by Fortis Life Sciences (1 mentions)

2 protocols using anti batf sc 100974

Immunoprecipitation and Immunoblot Analysis

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HEK293T cells were transfected with pcDNA3 encoding FLAG-tagged Jun family proteins and haemagglutinin (HA)-tagged BATF. After 24 h, the FLAG-tagged protein was immunoprecipitated using anti-FLAG (M2)-conjugated agarose (Sigma) and analyzed by immunoblot with the anti-FLAG (M2, Sigma) and anti-HA (16B12, Covance) antibodies. Endogenous proteins in T cell lysates were analyzed by immunoblot with anti-IRF4 (sc-6059, Santa Cruz), anti-BATF (sc-100974, Santa Cruz), anti-JunB (sc-46, Santa Cruz), anti-c-Jun (sc-1694 and sc-74543, Santa Cruz), anti-JunD (sc-74, Santa Cruz), and anti-β-actin (sc-47778, Santa Cruz) antibodies.

Yamazaki S., Tanaka Y., Araki H., Kohda A., Sanematsu F., Arasaki T., Duan X., Miura F., Katagiri T., Shindo R., Nakano H., Ito T., Fukui Y., Endo S, & Sumimoto H. (2017). The AP-1 transcription factor JunB is required for Th17 cell differentiation. Scientific Reports, 7, 17402.

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Nuclear Extraction and Immunoblot Analysis

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Nuclear extracts were prepared using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific). The antibodies used for the immunoblot analyses were anti-Chd4 (A301-081A; Bethyl), anti-Mta2 (sc-9447; Santa Cruz Biotechnology), anti-HDAC2 (ab12169; Abcam), anti-Rest (12C11-1B11; Caltech Protein Expression Center), anti-Ring1b (A302-869A; Bethyl), anti-LSD1 (ab17721; Abcam), anti-Runx1 (ab23980; Abcam), anti-Batf (sc-100974; Santa Cruz Biotechnology), anti-Bcl11b (ab18465; Abcam), anti-Vim (ab20346; Abcam), anti-Peg10 (ab181249; Abcam), and anti-Lamin B (sc-6217; Santa Cruz Biotechnology).
Because of the low recovery of Runx1 in co-immunoprecipitation with Bcl11b complexes from ILC2/b6 cells, independently made antibodies against Runx1 and Runx3 from the Weizmann Institute (Levanon et al., 2014 (link)) were also tested for immunoblotting. While showing higher Runx3 in total nuclear extracts of ILC2/b6 cells, co-immunoprecipitation results for Runx1 and Runx3 (data not shown) were similar to those shown for Runx1 using ab23980 in Fig. 4 D

Hosokawa H., Romero-Wolf M., Yang Q., Motomura Y., Levanon D., Groner Y., Moro K., Tanaka T, & Rothenberg E.V. (2019). Cell type–specific actions of Bcl11b in early T-lineage and group 2 innate lymphoid cells. The Journal of Experimental Medicine, 217(1), e20190972.

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