Apoptotic cells in SGs were identified using a TUNEL assay (ApopTag Plus in situ Apoptosis Kit; Chemicon Int., Temecula, CA, USA) at 16 weeks post-RI exposure. TUNEL-positive apoptotic cells at x400 and the numbers of cell in apoptosis were calculated in ten random sites32 (link).
Apoptag plus in situ apoptosis kit
Manufactured by Merck Group
Sourced in United States
The ApopTag Plus in situ Apoptosis Kit is a laboratory tool used to detect and analyze apoptosis, a programmed cell death process, in biological samples. The kit provides the necessary reagents and protocols to identify and visualize apoptotic cells through immunohistochemical techniques.
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Lab products found in correlation
2 protocols using apoptag plus in situ apoptosis kit
1
Apoptosis Quantification in Stress Granules
2
Apoptosis Assessment in Submandibular Gland
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Apoptosis in submandibular gland tissues was determined using an ApopTag Plus in situ Apoptosis Kit (Chemicon Int., Temecula, CA, USA). Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive cells were checked at a magnification of ×400, and numbers of TUNEL-positive cells were counted in 10 random fields. To detect apoptosis-related proteins, Western blotting was performed using anti-Bax (Cell Signaling Technology, Beverly, MA, USA) and anti- Bcl-xl (Santa Cruz Biotechnology, Santa Cruz, CA, USA) antibodies.
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