Log In Sign up
The largest database of trusted experimental protocols

Gipz lentiviral shrna library

Manufactured by Thermo Fisher Scientific
Visit Supplier

The GIPZ Lentiviral shRNA Library is a collection of lentiviral constructs designed for gene knockdown experiments. Each construct contains a short hairpin RNA (shRNA) sequence that targets a specific gene. The library is intended to enable researchers to systematically investigate gene function through loss-of-function studies.

Automatically generated - may contain errors

Lab products found in correlation

0.45μm membrane, by Corning (2 mentions) Transit x2 transfection reagent, by Mirus Bio (2 mentions)

Spelling variants of this product

GIPZ lentiviral shRNA (2 citations) Lentiviral shRNA library (2 citations)

2 protocols using gipz lentiviral shrna library

1

Selective Knockdown and Overexpression of Mef2c in rMSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
To selectively knockdown Mef2c in rMSC, lentiviruses of Mef2c/Control short hairpin RNA (shRNA) were generated by co-transfecting HEK-293T cells with 12μg shRNA shuttle vector (GIPZ Lentiviral shRNA Library, Open Biosystems, provided by Einstein shRNA Facility), 0.6 μg tat vector, 0.6 μg gag/pol vector, 1 μg vsv-g vector into HEK-293T cells using 45 μl TransIT-X2 transfection reagent (Mirus Bio). Supernatant was collected from HEK-293T cells, filtrated with 0.45 μm membrane (CORNING), and concentrated by ultracentrifugation. Concentrated virus preparations were used to transduce rMSC, and cells expressing the construct were selected by using 5.0µg/ml puromycin. To overexpress Mef2c in rMSC, lentiviruses were produced in HEK-293T cells as described above. rMSCs were transduced with concentrated lentiviral preparations, and Tomato+ cells sorted on day 5 post-transduction.
Nakahara F., Borger D.K., Wei Q., Pinho S., Maryanovich M., Zahalka A.H., Suzuki M., Cruz C.D., Wang Z., Xu C., Boulais P.E., Ma'ayan A., Greally J.M, & Frenette P.S. (2019). Engineering a haematopoietic stem cell niche by revitalizing mesenchymal stromal cells. Nature cell biology, 21(5), 560-567.
+ Open protocol
+ Expand
2

Selective Knockdown and Overexpression of Mef2c in rMSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
To selectively knockdown Mef2c in rMSC, lentiviruses of Mef2c/Control short hairpin RNA (shRNA) were generated by co-transfecting HEK-293T cells with 12μg shRNA shuttle vector (GIPZ Lentiviral shRNA Library, Open Biosystems, provided by Einstein shRNA Facility), 0.6 μg tat vector, 0.6 μg gag/pol vector, 1 μg vsv-g vector into HEK-293T cells using 45 μl TransIT-X2 transfection reagent (Mirus Bio). Supernatant was collected from HEK-293T cells, filtrated with 0.45 μm membrane (CORNING), and concentrated by ultracentrifugation. Concentrated virus preparations were used to transduce rMSC, and cells expressing the construct were selected by using 5.0µg/ml puromycin. To overexpress Mef2c in rMSC, lentiviruses were produced in HEK-293T cells as described above. rMSCs were transduced with concentrated lentiviral preparations, and Tomato+ cells sorted on day 5 post-transduction.
Nakahara F., Borger D.K., Wei Q., Pinho S., Maryanovich M., Zahalka A.H., Suzuki M., Cruz C.D., Wang Z., Xu C., Boulais P.E., Ma'ayan A., Greally J.M, & Frenette P.S. (2019). Engineering a haematopoietic stem cell niche by revitalizing mesenchymal stromal cells. Nature cell biology, 21(5), 560-567.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!