Poly d lysine coated 12 well plates

Manufactured by BD

Poly-D-lysine coated 12-well plates are a type of laboratory equipment used for cell culture. The plates are coated with the synthetic amino acid polymer poly-D-lysine, which promotes cell adhesion and growth.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Neurobasal media, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

12-well plates (126 citations) Poly-D-lysine (52 citations) Poly-D-Lysine coated plates (11 citations) Poly-D-lysine-coated (3 citations)

2 protocols using poly d lysine coated 12 well plates

Tau and MARK4 Regulation in 293T Cells

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293T cells were cultured at 37 °C in 5% CO₂ with Dulbecco’s modification of Eagle’s medium (DMEM) (Gibco/Life Technologies) with 10% fetal bovine serum (FBS) (Gibco/Life Technologies), 100 units/ml penicillin and 100 μg/ml streptomycin (Gibco/Life Technologies). For transfection, cells were plated in poly-D-lysine coated 12-well plates (BD) at 2.2 × 10⁵ cells/well. After 40 h incubation, each well were transfected with 1 μg of plasmid DNA encoding human 4R2N Tau and/or HA tagged human MARK4 (WT or KD) using lipofectamine 2000 (Life Technologies) according to manufacturer’s instruction. 24 h after transfection, cells were treated with AC, MB or OS at indicated final concentrations for 3 h at 37 °C. When MG132 was used to inhibit proteasomes, cells were pretreated with 500 nM MG132 for 1 h at 37 °C followed by drug treatment in the presence of MG132.

Sun W., Lee S., Huang X., Liu S., Inayathullah M., Kim K.M., Tang H., Ashford J.W, & Rajadas J. (2016). Attenuation of synaptic toxicity and MARK4/PAR1-mediated Tau phosphorylation by methylene blue for Alzheimer’s disease treatment. Scientific Reports, 6, 34784.

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Dissociation and Culture of Rat Hippocampal Neurons

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Dissociated hippocampal neurons were prepared from Sprague Dawley rat embryos on E18-E19 (Charles River Labs). Hippocampal tissue was dissected in ice-cold 1× HBSS. Hippocampal tissue was washed 3× with 20 mL of ice-cold HBSS and then digested at 37°C for 10min in 5 mL of HBSS with trypsin (Life Technologies). Hippocampal tissue were then washed 3 times with HBSS and gently triturated in 2 mL of HBSS and plated at 100,000 cells per well in poly-D-lysine-coated 12-well plates (BD Biosciences). Neurons were maintained in Neurobasal media (Life Technologies), supplemented with 1× B27 (Life Technologies), and 1% penicillin/streptomycin at 37°C with 5% CO₂ incubation.

Kim M.J., Biag J., Fass D.M., Lewis M.C., Zhang Q., Fleishman M., Gangwar S.P., Machius M., Fromer M., Purcell S.M., McCarroll S.A., Rudenko G., Premont R.T., Scolnick E.M, & Haggarty S.J. (2016). Functional Analysis of Rare Variants Found in Schizophrenia Implicates a Critical Role for GIT1-PAK3 Signaling in Neuroplasticity. Molecular psychiatry, 22(3), 417-429.

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