The following antibodies were used in this study: C-FOS (Cell Signaling, #2250, Clone#9F6, rabbit, 1:500); GFP (Abcam, ab13970, chicken, 1:1000); RAGC (Cell Signaling, #5466, rabbit, 1:500); TIPRL (Abcam, #ab70795, rabbit, 1:500); SHANK2 (Cell Signaling, #12218, rabbit, 1:500); SYNPO (Acris, #BM5086P, rabbit, 0.1 μg/ml); PSD95 (Cell Signaling, #2507, rabbit, 1:1000); HSP90 serum (gift from Dr Chung Wang, IMB, Taiwan, rabbit, 1:2000)21 (link); p-mTOR (Cell Signaling, #2448, rabbit, 1:500); mTOR (Cell Signaling, #2972S, rabbit, 1:500); S6K (Cell Signaling, #2708S, rabbit, 1:500); p-S6K70 T389 (Cell Signaling, #9205S, rabbit, 1:500); 4EBP (Cell Signaling, #9644S, rabbit, 1:500). The following reagents were obtained commercially: ProHance (Gadolinium contrast agent, Bracco Diagnostics, #111181); Vectastain Elite ABC kit (Vector Labs, #PK-6102); C21 (Tocris, #6422); OCT (Tissue-TeK, 4583); ZnSO4 (Sigma-Aldrich, #Z0251); 30 ppm zinc diet (Research Diets, D19410B). AAV vectors used in this study were: pAAV-CamKII-hM3D(Gq)-mCherry (Addgene, #50476); pAAV-CamKII-hM4D(Gi)-mCherry (Addgene, #50477)22 (link); pAAV-CAG-tdTomato (Addgene, #59462).
Tiprl
Manufactured by Abcam
Sourced in China
TIPRL is a protein that belongs to the TIP1 family. It functions as a GTPase-activating protein (GAP) for the small GTPase RNP24. TIPRL regulates the activity of RNP24, which is involved in protein trafficking and vesicle formation.
Automatically generated - may contain errors
Lab products found in correlation
Psd95, by Cell Signaling Technology (1 mentions)
Shank2, by Cell Signaling Technology (1 mentions)
C fos, by Cell Signaling Technology (1 mentions)
Paav cag tdtomato, by Addgene (1 mentions)
Znso4, by Merck Group (1 mentions)
P mtor, by Cell Signaling Technology (1 mentions)
Vectastain elite abc kit, by Vector Laboratories (1 mentions)
Prohance, by Bracco (1 mentions)
Evos m7000 imaging system, by Thermo Fisher Scientific (1 mentions)
2 protocols using tiprl
1
Antibody and Reagent Database for Neuroscience Research
2
Immunohistochemical Analysis of CD44 and TIPRL
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Xenografts or tissues were fixed with 4% paraformaldehyde and embedded with paraffin. Then, the sections were dewaxed in xylene and rehydrated in alcohol. Antigen retrieval was performed using sodium citrate, and then endogenous peroxidase was blocked. The sections were incubated with specific rabbit anti-human CD44 (1:100, Boster Biological Technology, China), and TIPRL (1:200, Abcam, USA) at 4 °C overnight. After incubation with secondary antibodies at 37 °C for 1 h, DAB was used for detection. The sections were observed under an EVOSTM M7000 Imaging System (Thermo Fisher Scientific, USA).
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