Rat cardiac H9c2 cells (ATCC, USA) were cultured in Dulbecco's modified Eagle's media (DMEM) (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany), which is supplemented with 10% fetal bovine serum (FBS; Takara Biotechnology Co., Ltd., Dalian, China), 100 ug/ml streptomycin (Takara Biotechnology Co., Ltd.), and 100 unit/ml penicillin (Takara Biotechnology Co., Ltd.) at 37°C in a humidified atmosphere with 5% CO2.
Rat cardiac h9c2 cells
Manufactured by American Type Culture Collection
Sourced in United States
Rat cardiac H9c2 cells are a well-established cell line derived from the ventricular tissue of an embryonic rat heart. They are commonly used as an in vitro model for cardiac muscle cells. The H9c2 cells exhibit characteristics similar to cardiomyocytes, making them a valuable tool for studying various aspects of cardiac biology and physiology.
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2 protocols using rat cardiac h9c2 cells
1
Culturing Rat Cardiac H9c2 Cells
2
Rat cardiac H9c2 cell culture protocol
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Rat cardiac H9c2 cells (ATCC Rockville, MD, USA) were cultured in DMEM supplemented with 10% heatinactivated FBS, 100 μg/mL streptomycin, and 100 U/ mL penicillin at 37°C in a humidified atmosphere (5% CO 2 -95% air). The cells were fed every 1-2 days, and detached using 0.05 trypsin/0.02% EDTA when they reached about 80-90% confluence. Then cells seeded at an appropriate density according to each experimental design, and the cells used were in the exponential phase of growth before exposure to drugs in all experiments.
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