Amh elisa kits

Serum levels of inhibin B, AMH, FSH, LH, estradiol (E2), PRL, progesterone, and T were evaluated. Levels of inhibin B and AMH were measured using inhibin B ELISA kits and AMH ELISA kits from Beckman Coulter Inc., which were described in the previous research (19 (link)). All serum measurements for the patients were performed in the same laboratory using the same assays. The assay was developed using a sequential application of sample, conjugated antibody, substrate, and amplifier, with the washing as instructed. Absorbance was read in a microplate reader at a wavelength of 450 nm, along with 620 nm used as a reference filter.
Sample concentrations of inhibin B were extrapolated from the standard curve using a cubic-cubic regression. The detection limit of the assay was 2.6 pg/mL, the intra- and inter-assay coefficients of variation were 3.8% and 5.2%, respectively. Samples with inhibin B concentrations less than the detection limit of the assay were assigned a value of 2.5 for analysis.
The levels of FSH, LH, E2, PRL, progesterone, and T were measured using a chemiluminescence-based immunometric assay on an ADVIA Centaur immunoassay system (Siemens Healthcare Diagnostics Inc., Tarrytown, NY, USA). The manipulation was performed following the manufacturer’s instructions.

Serum AMH level was measured using AMH ELISA kits (Beckman Coulter, Fullerton, CA, USA) following the manufacturer's directions. The minimum detectable concentration was 0.16 ng/mL, and the inter- and intra-assay coefficients of variation were 5.6 and 5.4%, respectively.
BRCA testing was conducted on peripheral blood using direct sequencing. When pathogenic variants were identified in the genetic tests, all mutations were interpreted utilizing the Human Gene Mutation Database (HGMD; http://www.hgmd.cf.ac.uk/), ClinVar (https://www.ncbi.nlm.nih.gov/clinvar), and Korea ONCOgene Research and Diagnosis (KONCORD; http://koncord.kr). Mutation nomenclatures from the Breast Cancer Information Core (BIC; http://research.nhgri.nih.gov/bic/) were used for the genetic test reports.