Flow cytometry analyses were performed in a BD Accuri cytometer. First, 20 µg of EV resuspension was bound to 20 µl of anti-CD63 antibody-coated magnetic beads (Life Technologies), as recommended by the manufacturer. Next, a second staining with anti-CD81-PE-conjugated antibody or anti-CD9-APC-conjugated antibody (Molecular Probes, Life Technologies, Eugene, OR, USA) was performed for 30 min at room temperature. The beads were then washed with PBD plus albumin 0.1% and were analyzed. At least 5000 events per treatment were counted.
Anti cd81 pe conjugated antibody
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Anti-CD81-PE-conjugated antibody is a laboratory reagent used for the detection and analysis of the CD81 protein. It is a fluorescently labeled antibody that specifically binds to the CD81 cell surface marker, allowing for its identification and quantification in various experimental applications.
Automatically generated - may contain errors
2 protocols using anti cd81 pe conjugated antibody
1
Extracellular Vesicle Immunophenotyping
2
Exosome Characterization by Flow Cytometry
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Flow cytometry analyses were performed in a BD Accuri cytometer. In order to confirm on which fractions exosomes were present, 100 ul of each fraction was bound with 5 ul of anti-CD63 antibody-coated magnetic beads (Life Technologies, Oslo, Norway), as recommended by the manufacturer. Next, anti-CD63 bound EVs were stained with an anti-CD81-PE-conjugated antibody and an anti-CD9-APC-conjugated antibody (Molecular Probes, Life Technologies, Eugene, OR, USA) for 30 min at room temperature. Beads were then washed with PBS 0.1% albumin and were analyzed. At least 10000 events per treatment were counted.
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