Lm6002

Lipidomic experiments were performed according to a procedure described previously⁴¹ (link). Briefly, for adipose tissue lipidomic analysis, approximately 20 mg of adipose tissue was homogenized in 200 μL of H₂O and then extracted with 1000 μL of precooled chloroform:methanol (2:1) solution containing LM6002 (Avanti Polar Lipids, Alabaster, AL, USA) as an internal standard. After centrifuged at 18,000×g for 5 min, the lower organic phase was collected to evaporate. For plasma lipidomic analysis, 200 μL of precooled chloroform:methanol (2:1) solution with LM6002 (Avanti Polar Lipids) was mixed with 50 μL of serum. Samples were centrifuged at 18,000×g for 5 min, and the lower organic phase was collected to evaporate. Then, the organic residue was re-dissolved in isopropanol:acetonitrile (1:1) solution for qualification. Eksigent LC100 coupled with an AB SCIEX Triple TOF 5600 system were used to analyze the samples. Peak extraction and integration were analyzed by Xcalibur 2.2 SP1.48 software (Thermo Fisher Scientific, Waltham, MA, USA).