Cs 2000i analyzer

After a fasting period of 12 hours, a peripheral venous blood sample was collected from all patients within the first 24 hours after admission. Biochemical indicators, such as serum albumin, total protein, total bilirubin, creatinine, and ALT, were determined using a Hitachi 7600 Clinical Analyzer (Hitachi, Tokyo, Japan), and the INR was analyzed using the coagulation method with a Sysmex CS-2000i Analyzer (Sysmex, Kobe, Japan). At baseline, for each patient, demographic and clinical data, including the age; sex; and complications of ascites, variceal bleeding, hepatorenal syndrome (HRS), or HE and clinical course, were obtained from the medical files and recorded in a specified liver disease document. In addition, the model for end-stage liver disease (MELD) score, Child–Pugh score, ALBI score, and serological indexes (HBsAg, HBeAg, anti-HBc, and HBV DNA levels) were collected at baseline.

The biochemistry variables were measured by using commercial kits Architect C16000 (Abbott Laboratories, Lake Forest, IL, USA). Homocysteine, ferritin and hsCRP were determined through chemical luminescence and immunoturbidimetric methods by Abbott Architect Automatic analyzer (Abbott Laboratories, Lake Forest, IL, USA).
ADAMTS13 activity was measured by LIFECODES ATS-13 Activity Assay kit (Immucor^® GTI Diagnostics, Waukesha, WI, USA). Based on fluorescence resonance energy transfer (FRET) technology, ATS-13 activity assay is for the quantitative measurement of ADAMTS13 protease activity. A synthetic fragment of the vWf protein is used as the substrate. vWF antigen and activity (vWF ristocetin cofactor) were measured by immunoturbidimetric assay kits (Siemens, Marburg, Germany) in Sysmex CS‐2000i analyzer (Sysmex Corporation, Kobe, Japan).