Retroviral plasmids containing tdTomato-tagged blue light-responsive TGF-β receptors (Addgene #118942) and iRFP682-tagged Smad2 (Addgene # 118943) were provided as a gift by Dr. Won Do Heo.24 (link) Transformed colonies of competent E. coli (C3019H; New England BioLabs) were selected, amplified, and purified (E.Z.N.A. Endo-Free Plasmid DNA Kit; Omega Bio-Tek). Omitting the retroviral backbone, lentiviral plasmids were designed using Benchling and SnapGene software and constructed using custom cloning service of Epoch Life Science. The sequences for the light-responsive TGF-β receptors and iRFP682-Smad2 were inserted separately into lentiviral backbones (pLV-EF1a-IRES-Neo; Addgene #85139). The lentiviral plasmids were similarly amplified and purified.
E z n a endo free plasmid dna kit
Manufactured by Omega Bio-Tek
The E.Z.N.A. Endo-Free Plasmid DNA Kit is a laboratory product designed to extract and purify plasmid DNA from bacterial cultures. It is intended for use in molecular biology applications that require high-quality, endotoxin-free plasmid DNA.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using e z n a endo free plasmid dna kit
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Engineered Light-Responsive TGF-β Signaling
2
Engineered Light-Responsive TGF-β Signaling
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Retroviral plasmids containing tdTomato-tagged blue light-responsive TGF-β receptors (Addgene #118942) and iRFP682-tagged Smad2 (Addgene # 118943) were provided as a gift by Dr. Won Do Heo.24 (link) Transformed colonies of competent E. coli (C3019H; New England BioLabs) were selected, amplified, and purified (E.Z.N.A. Endo-Free Plasmid DNA Kit; Omega Bio-Tek). Omitting the retroviral backbone, lentiviral plasmids were designed using Benchling and SnapGene software and constructed using custom cloning service of Epoch Life Science. The sequences for the light-responsive TGF-β receptors and iRFP682-Smad2 were inserted separately into lentiviral backbones (pLV-EF1a-IRES-Neo; Addgene #85139). The lentiviral plasmids were similarly amplified and purified.
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