Zo 1 antibody

Low endotoxin rhHSP70 and rhHSP27 were purchased from Enzo Life Sciences (Villeurbanne, Fr) and rabbit anti-HSP70 from ABR (AffinityBioReagent, ThermoFisher, Fr). Mouse anti-Hsc70, rabbit anti-phospho Cx43 (Ser368, Ser255, Ser262 and Tyr265) and probenecid were from Santa Cruz Biotech. Neutralizing anti-TLR4 and mouse anti-EGFR (ErB1) were from Abcam (Cambridge, UK). Mouse anti-TLR4, polymyxin B and gefitinib were from InvivoGen (Toulouse, Fr). Rabbit anti-P-EGFR (Tyr-1068) and antiphospho- and total Akt (Ser473) were from Cell Signaling (Danvers, USA) and mouse anti-Cx43 from Invitrogen. ZO-1 antibody was from Zymed (Invitrogen). DiL-C18, thapsigargin and fura-2/AM were from Molecular Probes. VER155008 and Gap26 (VCYDKSFPISHVR) from Tocris (McKinley, USA). Other chemicals were from Sigma-Aldrich.

Metformin (1,1-dimethylbiguanide hydrochloride) was purchased from Sigma-Aldrich (St. Louis, MO, USA). The [9,10(n)-³H] palmitic acid was purchased from NEN (Boston, MA, USA). Phosphorylated ACC (Ser79), phosphorylated p70S6K (Thr389), total ACC, and total p70S6K antibodies were acquired from Cell Signaling Technology (Danvers, MA, USA). The androgen receptor (AR) antibody was purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA), claudin 11 antibody was obtained from Invitrogen (Thermo Fisher Scientific, Waltham, MA, USA), ZO-1 antibody was acquired from Zymed Laboratories (Thermo Fisher Scientific), and β-tubulin antibody was purchased from Sigma-Aldrich. Tissue culture media and all other drugs were acquired from Sigma-Aldrich. Sprague–Dawley rats (Rattus norvegicus) were obtained from the Central Animal Facility of the Department of Veterinary Sciences (Universidad de Buenos Aires, Argentina).