When the nestlings were 13 days old, they were banded with metal rings. As a random subsample, three nestlings of the same age from each brood were designated for blood sampling (Kaliński et al. 2015 (Kaliński et al. , 2016)) . Blood samples of ca. 5 μl were taken from the ulnar vain of nestlings (98 nestlings from 33 clutches) to HemoCue cuvettes and analyzed immediately using portable HemoCue Hb 201+ photometer (HemoCue AB, Angelholm, Sweden) to measure hemoglobin concentration (g/l). To establish glucose concentration (mg/dl) in a second blood sample (5 μl), a portable HemoCue Glucose 201+ photometer was used (99 nestlings from 33 clutches). All field procedures were performed between 09:00 and 14:00 h.
Glucose 201 photometer
Manufactured by HemoCue
Sourced in Sweden
The HemoCue Glucose 201+ photometer is a compact and portable device designed for the measurement of glucose levels. It utilizes a cuvette-based system to analyze blood samples and provide quick and accurate glucose readings. The device is intended for professional use in healthcare settings.
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Lab products found in correlation
2 protocols using glucose 201 photometer
1
Measuring Nestling Hematology and Glucose
2
Measuring Haemoglobin and Glucose in Great Tit Nestlings
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When the nestlings of the great tit were 13–14-days-old, they were banded with individually numbered metal rings and measured (wing length, to the nearest 1 mm). A random subsample of three nestlings out of same-age nestlings from every brood were designated for blood sampling (Kaliński et al., 2011 ). Samples of 5 μl of blood were taken from the ulnar vein of nestlings to HemoCue cuvettes and analyzed in the field using a portable HemoCue Hb 201+ photometer to measure haemoglobin concentration (g/l). In the case of avian blood, this photometer shows haemoglobin values slightly higher than cyanomethaemoglobin spectrophotometry, as described by Eklom and Lill (2006) and Simmons and Lill (2006) . A portable HemoCue Glucose 201+ photometer (HemoCue AB, Angelholm, Sweden) was used to analogously establish glucose concentration (mg/dL) in a second sample of blood (also 5 μl). All field procedures were carried out between 9.00 and 14.00 h. During the experimental treatment, 65 broods of the great tit (comprising 556 nestlings) were banded and measured and blood samples from 190 nestlings taken.
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