Budesonide (99% purity), 5% w/v 2,4,6-trinitrobenzenesulfonic acid (TNBS) solution, sodium azide, dimethyl sulfoxide (DMSO), 1,4-Dithiothreitol (DTT), phosphate buffered saline (PBS), lipopolysaccharide (LPS), collagenase type VIII, and DNAse-I were purchased from Sigma-Aldrich (St. Louis, MO). Polyvinylpyrrolidone K30 (PVP) was purchased from TCI Chemicals (Portland, OR). Zirconium oxide beads (0.15 mm, 0.5 mm and 1.0 mm in diameter) were purchased from Next Advance (Averill Park, NY). Carboxylate-functionalized fluorescent polystyrene (PS) particles (200 nm red and 2 µm yellow-green) were purchased from Molecular Probes (Eugene, OR). Sterile cell strainers (100 µm) were purchased from Fisher Scientific (Hampton, NH). Pluronic F127 (Poloxamer 407) was obtained as a free sample from BASF corporation (Tarrytown, NY). Brefeldin A and fluorescent antibodies were obtained from eBioscience (San Diego, CA) or Biolegend (San Diego, CA).
Pluronic f127 poloxamer 407
Manufactured by BASF
Sourced in Germany
Pluronic F127 (Poloxamer 407) is a nonionic triblock copolymer composed of polyethylene oxide (PEO) and polypropylene oxide (PPO) blocks. It is a water-soluble, thermoreversible gelling agent used in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Sterile cell strainer, by Thermo Fisher Scientific (2 mentions)
Zirconium oxide beads, by Next Advance (2 mentions)
Budesonide, by Merck Group (1 mentions)
Collagenase type 8, by Merck Group (1 mentions)
Brefeldin a, by Thermo Fisher Scientific (1 mentions)
1 4 dithiothreitol, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Lipopolysaccharide, by Merck Group (1 mentions)
Sodium azide, by Merck Group (1 mentions)
Dnase 1, by Merck Group (1 mentions)
Polyvinylpyrrolidone k30, by Tokyo Chemical Industry (1 mentions)
Sodium dodecyl sulfate (sds), by Promega (1 mentions)
Ethyl ether, by Merck Group (1 mentions)
Phthalic anhydride, by Merck Group (1 mentions)
Dimethylformamide, by Carlo Erba (1 mentions)
Dimethyl sulfoxide (dmso), by Avantor (1 mentions)
Triethylamine, by Carlo Erba (1 mentions)
Trypan blue, by Thermo Fisher Scientific (1 mentions)
Succinic anhydride, by Merck Group (1 mentions)
4 protocols using pluronic f127 poloxamer 407
1
Isolation and Characterization of Immune Cells
2
Chitosan-based Nanoparticle Formulation
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Chitosan (molecular weight cut-off (MWCO) <50,000; degree of deacetylation, 90%) was purchased from Seafresh Chitosan Laboratory (Bangkok, Thailand). Pluronic® F127 (Poloxamer 407) was acquired from BASF (Lampertheim, Germany), and ethanol 99.7–100% was bought from BDH (East Yorkshire, UK). OX hydrochloride, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS), pyridine, phthalic anhydride, acetone, sodium acetate, succinic anhydride, ethyl ether, and 1,4-Dioxane were procured from Sigma-Aldrich (St Louis, MO, USA). Sodium dodecyl sulfate was purchased from Promega Corporation (Fitchburg, WI, USA). DMEM, PBS, trypsin-EDTA, FBS, Trypan Blue, Vybant MTT cell proliferation kit, and anti-HER2, were obtained from Invitrogen (Waltham, MA, USA). Dimethyl sulfoxide (DMSO) was bought from Amresco (Cleveland, OH, USA). Triethylamine, dimethylformamide (DMF), and Hydrazine monohydrate were acquired from Carlo ERBA (Val-de-Reuil, France). Succinic anhydride was obtained from Fluka (Munich, Germany). Micro BCATM Protein Assay Kit was purchased from Thermo Scientific (Waltham, MA, USA).
3
Moxifloxacin-based Antimicrobial Ocular Formulation
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MOX (>99% purity) was purchased from LC Labs (Woburn, MA). Formalin, PAM, sodium oleate, sodium laurate, and sodium chloride were purchased from Sigma (St. Louis, MO). High‐performance liquid chromatography (HPLC)‐grade acetonitrile, sodium dihydrogen phosphate, and triethylamine were obtained from VWR International (Radnor, PA). Vigamox® (0.5% moxifloxacin eye drops, Alcon Laboratories Inc., Fort Worth, TX) was obtained from the Johns Hopkins Pharmacy. Zirconium oxide beads (0.5 mm in diameter) were purchased from Next Advance (Averill Park, NY). Sterile cell strainers (100 μm) were purchased from Fisher Scientific (Hampton, NH). Pluronic F127 (Poloxamer 407) was obtained as free samples from BASF Corporation (Tarrytown, NY). Tryptic soy agar, tryptic soy broth, sterile cotton‐tipped applicator, sterile disposable petri plates, and sterile phosphate‐buffered saline (PBS) and 20‐G needles were purchased from Thermo Fisher Scientific (Walthum, MA). Staphylococcus aureus (ATCC 25923) was obtained from ATCC (Manassas, VA). Proparacaine eye drops were obtained from Bausch and Lomb Inc. (Tampa, FL).
4
Polymeric Biomaterial Formulations
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Gantrez® S-97 (Mw = 1500.000 Da), a copolymer of methyl vinyl ether and maleic acid polymers, was provided by Ashland (Surrey, UK). Poly (ethylene glycol) (PEG, Mw = 10,000 Da), fluorescein sodium (FS) MW 376.27 Da, polyvinylpyrrolidone (PVP K32, MW 40,000 Da; PVP K90, MW 360,000 Da), and methylene blue were all purchased from Sigma–Aldrich (Dorset, UK). Pluronic® F-127 (poloxamer 407) (MW 12500 Da), Pluronic® F-87 (poloxamer 237) (MW 7700 Da), and Pluronic® F108 (MW 14600) were purchased from BASF Chemical Company, Ludwigshafen, Germany. All other chemicals and solvents used were of pure analytical grade.
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