Cells were washed twice in phosphate-buffered saline, counted, and re-suspended in FACS buffer (3% FBS in PBS containing 0.01% NaN3). Cells (1 × 106 cells/samples) for phenotypic analyses were stained using the indicated fluorochrome-conjugated antibody for 30 min on ice. Cells were washed with FACS buffer, and appropriate isotype controls were used in all cases. Typical forward- and side-scatter gates for flow cytometric analyses were set to exclude dead and aggregated cells. A total of 104 events in the gate were collected and analyzed using Accuri C6 and software (BD Biosciences, USA). The anti-CD23, anti-CD16/32 and anti-F4/80 antibodies (Abs) used in flow cytometric analyses were obtained from eBioscience (USA).
Accuri c6 and software
Manufactured by BD
Sourced in United States
The Accuri C6 is a benchtop flow cytometer designed for routine flow cytometry analysis. It provides automated sample acquisition, analysis, and data management capabilities. The associated software enables users to collect, analyze, and share flow cytometry data.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using accuri c6 and software
1
Flow Cytometric Analysis of Immune Cells
2
Multiparametric Flow Cytometry Analysis of Lymphocytes
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Single cell suspensions of the thymus, spleen, and lymph nodes were lysed of Red Blood Cells, the cells were resuspended in 100 μl of 2% BSA in PBS incubated on ice for 10 min, followed by biotinylated mAb, FITC-streptavidin, and PE-, Precpcy5.5-, or APC-labeled mAb were added to the cells which were then incubated on ice water bath at 4 °C for 45 min, and washed with PBS containing 2% BSA. A total of 104 cells events in the gate were collected and analyzed using Fortessa or Accuri C6 and software (BD. Inc). All fluorescence-labeled antibodies, including anti-CD3, −CD4, −CD8, −CD25, −CD69, −CD44, −CD62L, −TCRα, −TCRβ, −CD28, −CD27, 4-1BB and CTLA-4 were obtained from BD Biosciences. Apoptotic cells were determined by Annexin V and PI staining using an Annexin V-FITC kit (BD. Inc) following the manufacturer’s protocol.
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