Ginsenoside Rg1 (Rg1) was purchased from the Nanjin Jingzhu Biotechnology Company. Dimethyl sulfoxide (DMSO), verapamil, 2-APB and GdCl3 were obtained from Sigma-Aldrich (St. Louis, MO, USA). NPS2143(CaSR inhibitor) was obtained from Selleck Chemicals (Houston, TX, USA). IP3R antibody was purchased from AbSci (Baltimore, MD, USA). NFAT-3 antibody, TGF-β1 antibody and Smad2 antibody were purchased from Abcam (Cambridge, MA, USA). CaSR antibody, CaN antibody, type I collagen antibody, type III collagen antibody and β-actin antibody were obtained from Proteintech Biotechnology.
Type 1 collagen antibody
Manufactured by Proteintech
Sourced in United States
Type I collagen antibody is a primary antibody used for the detection and analysis of type I collagen, a major structural protein found in various connective tissues such as bone, skin, and tendon. This antibody can be utilized in techniques like immunohistochemistry, Western blotting, and ELISA to identify and quantify type I collagen.
Automatically generated - may contain errors
Lab products found in correlation
Gdcl3, by Merck Group (1 mentions)
Nps2143, by Selleck Chemicals (1 mentions)
Smad2 antibody, by Abcam (1 mentions)
2 apb, by Merck Group (1 mentions)
Tgf β1 antibody, by Abcam (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Verapamil, by Merck Group (1 mentions)
β actin antibody, by Proteintech (1 mentions)
Pvdf transfer membrane, by Merck Group (1 mentions)
2 protocols using type 1 collagen antibody
1
Ginsenoside Rg1 Signaling Pathway
2
Western Blotting Analysis of Wnt3, β-Catenin, and Collagen
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells or tissues were sonicated in RIPA buffer containing protease inhibitors. The supernatants were collected after centrifugation. Proteins of an equal concentration were separated on 10% SDS-PAGE and transferred to a PVDF transfer membrane (Millipore Corporation, Temecula, CA). The membranes were blocked in TBS containing 0.1% Tween-20 and 5% nonfat dry milk for 90 min, followed by an overnight incubation at 4°C with Wnt3 antibody (Proteintech, USA) at a 1 : 1000 dilution, β-catenin antibody (R&D, USA), and type I collagen antibody (Proteintech, USA) at a 1 : 250 dilution. After a rinse in TBST, the membranes were incubated for 1 h with a horseradish peroxidase-conjugated secondary antibody against rabbit or mouse IgG (Dako, Denmark) in a 1 : 5000 dilution. Then, they were rinsed with TBST followed by SuperSignal West Pico Chemiluminescent Substrates (Pierce, USA) for detecting the blots. The densities of the bands were analyzed by the Gel-Pro Analyzer. The expression of β-actin was used as an internal control.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!