Mv diluent

Manufactured by Flow Tech

The MV-Diluent is a laboratory equipment designed to dilute samples for analysis. It provides a precise and consistent method for diluting various types of samples, ensuring accurate measurements and reliable results.

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Lab products found in correlation

Mv 112, by Flow Tech (1 mentions) Skyscan 1276, by Bruker (1 mentions) Mv curing agent, by Flow Tech (1 mentions)

2 protocols using mv diluent

Microfil Perfusion and Imaging of Tumor Vasculature

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Tumor-bearing mice were intracardially perfused with Microfil injection compounds MV-112 and MV-Diluent (Flow Tech, Carver, MA). Before Microfil perfusion, mice were anesthetized, perfused with PBS then 4% paraformaldehyde. Four days after Microfil perfusion, tumors were excised, embedded in 1% agarose, and scanned using a non–gantry-based SkyScan 1172 μCT system (SkyScan, Kontich, Belgium). Tumors were scanned 360° around the vertical axis, in rotation steps of 0.1° with an AU filter of 0.25 μm. Generated isotropic pixel sizes were 6.6 μm. Stacks were re-constructed using ring reduction and 30% beam hardening correction. Images were thresholded using the Yen function in Image J then visualized using Imaris software.

Steinberger K.J., Forget M.A., Bobko A.A., Mihalik N.E., Gencheva M., Roda J.M., Cole S.L., Mo X., Hoblitzell E.H., Evans R., Gross A.C., Moldovan L., Marsh C.B., Khramtsov V.V, & Eubank T.D. (2020). Hypoxia-Inducible Factor alpha subunits regulate Tie2-expressing macrophages that influence tumor oxygen and perfusion in murine breast cancer. Journal of immunology (Baltimore, Md. : 1950), 205(8), 2301-2311.

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Lung Vascular Imaging via Microfil Perfusion

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Mice were anesthetized with avertin and euthanized by cutting off the abdominal aorta. The lung was perfused with 0.9% saline through the right ventricle, followed by perfusion using 5 mL of previously prepared Microfil (2.5 mL MV Compound +2.5 mL MV Diluent +4% MV-Curing Agent, Flow Tech Inc, MV-120). The lung was left for 30 min for the contrast agent to polymerize and was harvested and fixed in a 10% formalin solution. The micro vessels in lungs were imaged using a micro-CT SkyScan1276 (BRUKER), with settings as follows: 50 kV, 180 μA, pixel size 3.05 μm, 0.2-degree rotation step, and 180-degree rotation. The CT section images were captured using a CTAn software to reconstruct three-dimensional images.

Yan K., Zhang J., Yin W., Harding J.N., Ma F., Wu D., Deng H., Han P., Li R., Peng H., Song X, & Kang Y.J. (2022). Transcriptomic heterogeneity of cultured ADSCs corresponds to embolic risk in the host. iScience, 25(8), 104822.

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