The details of the reagents and cell culture are provided in the Supplemental Materials . S-2-(4-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid (p-SCN-Bn-NOTA) was purchased from Macrocyclics, Inc. (Plano, TX, USA). Chelex 100 resin (50–100 mesh) was purchased from Sigma-Aldrich (St. Louis, MI, USA). PD-10 desalting columns were purchased from GE Healthcare (Chicago, IL, USA). Prdx1 was acquired from Sinobiological (Beijing, China). All other reaction buffers and chemicals were purchased from Thermo Fisher Scientific (Fisher store in Stanford, USA).
Prdx1
Manufactured by Sino Biological
Sourced in China
Prdx1 is a protein that functions as an antioxidant enzyme. It plays a role in reducing hydrogen peroxide levels in cells.
Automatically generated - may contain errors
Lab products found in correlation
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
Pd 10 desalting column, by GE Healthcare (1 mentions)
Chelex 100 resin 50 100 mesh, by Merck Group (1 mentions)
S 2 4 isothiocyanatobenzyl 1 4 7 triazacyclononane 1 4 7 triacetic acid p scn bn nota, by Macrocyclics (1 mentions)
Gapdh, by Sino Biological (1 mentions)
Steponeplus real time pcr system, by Thermo Fisher Scientific (1 mentions)
2 protocols using prdx1
1
Radiolabeling of NOTA-Prdx1 Conjugate
2
Quantification of Antioxidant Gene Expression
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Total RNA was isolated from the cells using TRIzol Reagent (Thermo Scientific, MA). qPCR analysis was performed using standard procedures on a StepOnePlus Real-Time PCR System (Applied Biosystems, CA). PCR reactions were performed in triplicate and the relative amount of cDNA was calculated by the comparative CT method using RPS21 as an endogenous control. RT-PCR was performed in at least three biological replicates. The Taqman probe for NQO1 (Cat: HP101580), PRDX1 (Cat: HP101099), TXN (Cat: HP100418), FTL (Cat: HP104808), and GAPDH (Cat: HP100003) were purchased from Sino Biological Inc. (Beijing, China). The primers for Human GCLC: 5′-ATGTGGACACCCGATGCAGTATT-3′ (forward) and 5′-TGTCTTGCTTGTAGTCAGGATGGTTT-3′ (reverse) and HMOX: 5′-AACAAGCAGAACCCAGTCTATGC-3′ (forward) and 5′-AGGTAGCGGGTATATGCGTGGGCC-3′ (reverse). Gene expression was normalized to housekeeping gene expression (GAPDH).
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