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Anti cd4 fitc

Manufactured by Bio-Rad
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Anti-CD4-FITC is a fluorescently labeled monoclonal antibody that binds specifically to the CD4 cell surface receptor. It is used for the identification and enumeration of CD4-positive cells in flow cytometry applications.

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Lab products found in correlation

Ca2 ionomycin, by Merck Group (2 mentions) Brefeldin a, by Bio-Rad (2 mentions) Anti cd8 pacific blue, by Bio-Rad (2 mentions) Cytofix cytoperm kit, by BD (2 mentions) Golgiplug, by BD (2 mentions) Anti cd3, by Bio-Rad (1 mentions) Facsaria 3, by BD (1 mentions)

Close spelling variants of this product

FITC-conjugated anti-bovine CD4 FITC-conjugated anti-bovine CD4 antibody (CC8; Mouse anti-bovine CD4: FITC; Mouse anti‐cat CD4:FITC Anti-CD4

3 protocols using anti cd4 fitc

1

Quantifying IFN-γ Production in T Cells

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For assaying IFNγ production, 4 × 105 PBMCs were stimulated for 5 h in the presence of 2 ng/mL PMA, 4 μg/mL Ca2+ ionomycin (Sigma-Aldrich), and Brefeldin A (BD GolgiPlug; BD Biosciences, San Jose, CA, USA) adapted from described stimulation conditions [32 (link)] or overnight with 40 μg/mL T. cruzi lysate at 37 °C. Brefeldin A was added 5 h prior to end of incubation, and the cells were stained with anti-CD8-Pacific Blue and anti-CD4-FITC (AbD Serotec, Raleigh, NC, USA) followed by intracellular staining with anti-bovine IFN-γ AF647 (AbD Serotec) according to the BD Cytofix/Cytoperm kit (BD Biosciences). Samples were fixed in 2% formaldehyde prior to analysis by flow cytometry.
Hartley A.N., Cooley G., Gwyn S., Orozco M.M, & Tarleton R.L. (2014). Frequency of IFNγ-producing T cells correlates with seroreactivity and activated T cells during canine Trypanosoma cruzi infection. Veterinary Research, 45(1), 6.
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2

Canine Vaccine IFNγ Response Assay

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For assaying IFNγ levels, 4×105 PBMCs were stimulated for 5hr in the presence of 2ng/mL phorbol 12-myristate 13-acetate (PMA), 4µg/mL Ca2+ ionomycin (Sigma-Aldrich), and brefeldin A (BD GolgiPlug; BD Biosciences) (Pedersen et al., 2002 ). For polyclonal activation, PBMCs were plated with 15µg/mL anti-CD3 (AbD Serotec) or diluted whole vaccine antigens, incubated overnight at 37°C and brefeldin A added 5hr prior to end of incubation. Canine vaccines were IMRAB 3TF (Rabies; Merial, Athens, GA, USA), Duramune 5 (Canine distemper-Adenovirus Type 2-Parainfluenza-Parvovirus (DAPP); Fort Dodge Animal Health, Fort Dodge, IA, USA), and Leptovax 4 (Leptospirosis bacterial extract; Fort Dodge Animal Health) vaccines. Cells were stained with anti-CD8-Pacific Blue and anti-CD4-FITC (AbD Serotec) followed by intracellular staining with anti-bovine anti-IFN-γ AF647 (AbD Serotec) according to the BD Cytofix/Cytoperm kit (BD Biosciences).
Hartley A.N, & Tarleton R.L. (2015). CHEMOKINE RECEPTOR 7 (CCR7)-EXPRESSION AND IFNγ PRODUCTION DEFINE VACCINE-SPECIFIC CANINE T CELL SUBSETS. Veterinary immunology and immunopathology, 164(0), 127-136.
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3

Phenotyping Chicken Immune Cells

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Thymus and spleen tissues were prepared to obtain single-cell suspensions (2 × 106 cells) as previously described [48 (link)]. Immunofluorescence staining was performed with anti-chicken directly-conjugated MAbs: anti-CD4 FITC, anti-CD8 PE and anti-chT1 FITC (AbD Serotec, Ltd., Oxford, UK). Cell suspensions were added to PBS wash buffer containing 2 % bovine serum albumin and diluted antibodies for 30 min at 4 °C, followed by washing and resuspension in PBS containing 1 % paraformaldehyde. All cell populations were assessed using a FACSAria III (BD Biosciences, San Jose, CA, USA).
Huang H., Liu A., Wu H., Ansari A.R., Wang J., Huang X., Zhao X., Peng K., Zhong J, & Liu H. (2016). Transcriptome analysis indicated that Salmonella lipopolysaccharide-induced thymocyte death and thymic atrophy were related to TLR4-FOS/JUN pathway in chicks. BMC Genomics, 17, 322.
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