Log In Sign up
The largest database of trusted experimental protocols

Prkaa1tm1.1sjm j

Manufactured by Jackson ImmunoResearch
Visit Supplier

Prkaa1tm1.1Sjm/J is a mouse strain with a targeted mutation in the Prkaa1 gene, which encodes the AMP-activated protein kinase (AMPK) alpha 1 catalytic subunit. This mouse strain can be used for research purposes to study the role of AMPK in various biological processes.

Automatically generated - may contain errors

Lab products found in correlation

Fvb tg gfap cre 25mes j, by Jackson ImmunoResearch (2 mentions) B6 cg tg camk2a cre t29 1stl j, by Jackson ImmunoResearch (1 mentions) Rosamt mg, by Jackson ImmunoResearch (1 mentions) C57bl 6j mice, by Jackson ImmunoResearch (1 mentions)

5 protocols using prkaa1tm1.1sjm j

1

Conditional AMPK Deletion in Myeloid Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
LysMCre mice (B6.129P2-Lyz2tm1(cre)lgo/J; Jackson Laboratories, stock no. 004781), which harbor a nuclear localized Cre recombinase inserted into the first coding ATP of the lysozyme 2 gene, were mated with AMPKα1f/f mice (Prkaa1tm1/1Sjm/J: Jackson Laboratories, stock no. 014141), which possess loxP sites flanking exon 3 of the AMPKα1 gene. AMPKα1f/f mice were backcrossed to C57BL/6J mice for at least 10 generations. Successful mating was confirming by PCR genotyping and by flow cytometry analysis of AMPK expression in myeloid cells. Littermates were used in all experimental procedures
Vaibhav K., Braun M., Khan M.B., Fatima S., Saad N., Shankar A., Khan Z.T., Harris R.B., Yang Q., Huo Y., Arbab A.S., Giri S., Alleyne CH J.r., Vender J.R., Hess D.C., Baban B., Hoda M.N, & Dhandapani K.M. (2018). Remote ischemic post-conditioning promotes hematoma resolution via AMPK-dependent immune regulation. The Journal of Experimental Medicine, 215(10), 2636-2654.
+ Open protocol
+ Expand
2

Generation of PERK cKO Mice

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
All mice are housed in a barrier facility dedicated to transgenic mice at Wake Forest School of Medicine. Randomization was not used in animal studies. The facility operates in accordance with standards and policies of the US Department of Agriculture’s Animal Welfare Information Center (AWIC), and the NIH Guide for Care and Use of Laboratory Animals. The facility is kept on a 12 h light/dark cycle, with a regular feeding and cage-cleaning schedule. Mice of both sexes were used. The following mice were purchased from the Jackson Laboratory (Bar Harbor, ME): B6.Cg-Tg (Camk2a-cre)T29-1Stl/J (Camk2a-cre mice), stock No. 005359; Prkaa1tm1.1Sjm/J (loxP-flanked Prkaa1 mice), stock No. 014141; Prkaa2tm1.1Sjm/J (loxP-flanked Prkaa2 mice), stock No. 014142. Generation of PERK cKO mice was as described [27 (link)]. The genotypes were verified by polymerase chain reaction (PCR). Mice of 3–6-month old were used for all experiments. Investigators were not blind to the group allocation during the experiments.
Yang W., Zhou X., Zimmermann H.R, & Ma T. (2020). Brain-specific suppression of AMPKα2 isoform impairs cognition and hippocampal LTP by PERK-mediated eIF2α phosphorylation. Molecular psychiatry, 26(6), 1880-1897.
+ Open protocol
+ Expand
3

Animal Models for Neurological Research

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Adult male Sprague-Dawley rats (weight range 225–300 g, Harlan Laboratories, Indianapolis, IN) or male mice (weight range 25–35 g) were used. FVB-Tg(GFAP-cre)25Mes/J, 21 (link) Prkaa1tm1.1Sjm/J, 22 (link) and GFP-GFAP 23 (link) mice were purchased from Jackson Laboratories (Bar Harbor, MN). All experiments were approved by the Institutional Animal Care and Use Committee at the University of Georgia (Athens, Georgia) and were fully compliant with the National Institutes of Health Guidelines for the Use and Care of Laboratory Animals.
Maixner D.W., Yan X., Gao M., Yadav R, & Weng H.R. (2015). Adenosine Monophosphate-activated Protein Kinase Regulates Interleukin-1β Expression and Glial Glutamate Transporter Function in Rodents with Neuropathic Pain. Anesthesiology, 122(6), 1401-1413.
+ Open protocol
+ Expand
4

Tracing Lgr5+ Epithelial Cells in Murine Gut

Check if the same lab product or an alternative is used in the 5 most similar protocols
C57BL/6 J mice were purchased from Jackson Laboratory (#000664, Bar Harbor, ME, USA). Mice harbouring an Lgr5-EGFP-IRES-creERT2 (Lgr5) allele (#008875, Jackson Lab) were crossed with ROSAmT/mG (#007576, Jackson Lab) mice to obtain conditional knockout mice (Lgr5mT/mG). Epithelial stem cells in ex vivo gut from Lgr5mT/mG mice will convert cellular fluorescent protein from red to green signal in Lgr5-positive cells and their derived cells upon induction of 4-hydroxytamoxifen. The double-fluorescent ex vivo gut facilitated the tracing of epithelial movement and reorganization of Lgr5-derived cells. Mice with AMPKα1-floxed gene (Prkaa1tm1.1Sjm/J, #014141, Jackson Lab) were cross-bred with tamoxifen-inducible Cre mice (129-Gt(ROSA)26Sortm1(cre/ERT)Nat/J, #004847, Jackson Lab) as previously described to obtain AMPKα1 conditional knockout (KO) mice [27 (link)]. In response to 4-hydroxytamoxifen, AMPKα1 can be deleted in all tissues of AMPKα1 KO mice. Embryonic (E) staging was started from the morning when a copulatory plug was observed and counted as embryonic day 0.5 (E0.5). Pregnant mice at E13.5 were euthanized for ex vivo gut preparation.
Sun X., Fu X., Du M, & Zhu M.J. (2018). Ex vivo gut culture for studying differentiation and migration of small intestinal epithelial cells. Open Biology, 8(4), 170256.
+ Open protocol
+ Expand
5

Mice Strain Characterization for Neuroscience Research

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Male and female mice (weight range 25-40 g) were used. FVB-Tg(GFAP-cre)25Mes/J [52 (link)] and Prkaa1tm1.1Sjm/J [53 (link)] mice were purchased from Jackson Laboratories. All experiments were approved by the Institutional Animal Care and Use Committee at the University of Georgia and were fully compliant with the National Institutes of Health Guidelines for the Use and Care of Laboratory Animals.
Maixner D.W., Yan X., Hooks S.B, & Weng H.R. (2016). AMPKα1 knockout enhances nociceptive behaviors and spinal glutamatergic synaptic activities via production of reactive oxygen species in the spinal dorsal horn. Neuroscience, 326, 158-169.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!