Cell protein extracts were prepared using M-Per Mammalian Protein Extraction Reagent (Pierce, Rockford, IL) according to the manufacturer’s instructions. Total protein was fractionated by SDS-PAGE and transferred onto PVDF membranes. The following primary antibodies were used: PD-L1 (17952-1-AP, ProteinTech), SOX6 (NBP1-85811, Novus), WNK2 (07-2261, Millipore), BTG3 (ab112938, Abcam), RBSP3 (ab106973, Abcam), active β-catenin (05-665, Millipore), OCT4 (ab19857, Abcam). p-AKT (sc-293125), p-ERK1/2 (sc-16982), total β-catenin (sc-7963), ERK1/2 (sc-514302), PTEN (sc-7974), cyclin D1 (sc-8396), p21 (sc-6246), and GAPDH (sc-47724) were obtained from Santa Cruz Biotechnology. GAPDH was analyzed to show equal protein loading. Blots were developed with the enhanced chemiluminescence blotting analysis system (Amersham Pharmacia Biotech, Buckinghamshire, UK). Immunoblot images were digitized and quantified using the ImageJ software. Results were expressed as a relative ratio of PD-L1 to GAPDH and the PD-L1/GAPDH ratio in normal cells was set as 1.
Pd l1 17952 1 ap
Manufactured by Proteintech
Sourced in China
PD-L1 (17952-1-AP) is a primary antibody product offered by Proteintech. It is designed to detect the expression of the PD-L1 (Programmed cell death 1 ligand 1) protein in various samples.
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Lab products found in correlation
Enhanced chemiluminescence blotting analysis system, by Cytiva (1 mentions)
Active β catenin, by Merck Group (1 mentions)
Ab19857, by Abcam (1 mentions)
Sc 47724, by Santa Cruz Biotechnology (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
Hluga150cs03, by Shanghai Outdo Biotech Co. (1 mentions)
Itgb1, by Cell Signaling Technology (1 mentions)
Hluga180su04, by Shanghai Outdo Biotech Co. (1 mentions)
Envision kit, by Agilent Technologies (1 mentions)
2 protocols using pd l1 17952 1 ap
1
Immunoblot Analysis of PD-L1 and Associated Proteins
2
Immunohistochemical Analysis of Lung Adenocarcinoma
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Microarrays of human lung adenocarcinoma tissues (HLugA150CS03 and HlugA180Su04) were obtained from Shanghai Outdo Biotech Co., Ltd. (Shanghai, China). Samples underwent USP51, PD‐L1 (17952‐1‐AP, Proteintech, Wuhan, Hubei, China) and ITGB1 (34971T, Cell Signaling Technology) antibody staining for IHC assays with the Envision Kit (Dako) in accordance as per kit protocols. Several pathologists then blindly scored the immunostaining, as described previously [24 (link)]. This protocol received ethical approval from the Nankai University (NKUIRB2021010).
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