Fresh leaves of Toona sinensis were collected in October 2010 from Fuzhou, Fujian Province, China. The plant was identified by Associate Professor Cheng Zi Yang of the Pharmaceutical College, Fujian University of Traditional Chinese Medicine. A voucher specimen was deposited at Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine.
Anhydrous sodium sulfate and n-hexane were purchased from Sinopharm Chemical Reagent, Co., Ltd. (Shanghai, China). Dimethyl sulfoxide (DMSO), 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyl tetrazolium bromide (MTT), paraformaldehyde (PFA) and the antibiotic oxacillin (OXA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS), Dulbecco’s Modified Eagle Medium (DMEM), trypsin, and penicillin–streptomycin were obtained from Hyclone Laboratories, INC. (Logan, UT, US). Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis detection kit was purchased from Nanjing KeyGen Biotech CO., Ltd. (Nanjing, China). Hoechst 33324 staining kit was from Beyotime Institute of Biotechnology (Beijing, China). Caspase-3 antibody was from GeneTex Inc. (Irvine, CA, USA). Bcl-2, Bax and β-actin antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). n-Alkanes C8-C40 were purchased from AccuStandard, Inc. (New Haven, CT, USA). All other chemicals were of the highest purity available in China.
Anhydrous sodium sulfate and n-hexane were purchased from Sinopharm Chemical Reagent, Co., Ltd. (Shanghai, China). Dimethyl sulfoxide (DMSO), 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyl tetrazolium bromide (MTT), paraformaldehyde (PFA) and the antibiotic oxacillin (OXA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS), Dulbecco’s Modified Eagle Medium (DMEM), trypsin, and penicillin–streptomycin were obtained from Hyclone Laboratories, INC. (Logan, UT, US). Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis detection kit was purchased from Nanjing KeyGen Biotech CO., Ltd. (Nanjing, China). Hoechst 33324 staining kit was from Beyotime Institute of Biotechnology (Beijing, China). Caspase-3 antibody was from GeneTex Inc. (Irvine, CA, USA). Bcl-2, Bax and β-actin antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). n-Alkanes C8-C40 were purchased from AccuStandard, Inc. (New Haven, CT, USA). All other chemicals were of the highest purity available in China.