Rabbit antilamin b polyclonal antibody

Western blot analyses were performed in PBMC or nuclear extracts homogenates as described previously [28 (link)]. Lysate proteins were fractionated by SDS-PAGE and Western blotting was performed using the corresponding primary antibodies. Antibodies against TLR4 (96 kDa), MyD88 (33 kDa), p65 (65 kDa), pERK (42–44 kDa), TBK1 (80 kDa), IRF3 (50 kDa), protein C-reactive (PCR) (30 kDa), and TNFα (27 kDa) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA); antibodies against CD14 (40 kDa), TRAF6 (55 kDa), TRIF (66 kDa), and IKKi/IKKε (80 kDa) were purchased from Abcam (Cambridge, UK); and antibodies against pIKK (85–87 kDa), pIκB (40 kDa), p38 (43 kDa), pP38 (43 kDa), and pIRF3 (45–55 kDa) were purchased from Cell Signaling Technology (Beverly, MA). Bound antibody was detected by enhanced chemiluminescence.
Equal loading of protein was demonstrated by probing the membranes with a rabbit antilamin-B polyclonal antibody (Santa Cruz Biotechnology) (67 kDa) or rabbit anti-β-actin polyclonal antibody (Sigma-Aldrich) (42 kDa). The density of the specific bands was quantified with an imaging densitometer (Image J, National Institute of Health, Bethesda, MD).

Baicalin (pure, 98%) was purchased from Shanghai Xin Yu Biological Technology Co., Ltd (Shanghai, China). LPS (E. coli, serotype 055: B5) was purchased from Sigma (St. Louis, MO, USA). ELISA kits of IL-1β, TNF-α, and IL-6 were purchased from BioLegend (Camino Santa Fe, CA, USA), MPO and NO assay kits were obtained from the Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Rabbit anti-phospho-IkBα polyclonal antibody, rabbit anti-phospho-p65 polyclonal antibody and rabbit anti- p65 polyclonal antibody were purchased from Sangon Biotech Co., Ltd (Shanghai, China). Rabbit anti-TLR4 polyclonal antibody was obtained from Santa Cruz Biotechnology (Dallas, TX, USA). Rabbit anti- β-actin polyclonal antibody was obtained from univ Biotechnology (Shanghai, China). Rabbit anti-lamin-B polyclonal antibody was obtained from Santa Cruz Biotechnology. And HRP-conjugated goat anti-rabbit IgG was obtained from ZSGE-BIO (Beijing, China). SYBR Green PCR Master Mix was purchased from Toyobo Life Science Co., Ltd (Shanghai, China). And M-MULV cDNA reverse transcription kit was purchased from Sangon Biotech Co., Ltd (Shanghai, China).