Anti erα mc20

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-ERα (MC20) is a mouse monoclonal antibody that recognizes the estrogen receptor alpha (ERα) protein. It can be used for the detection of ERα in various applications.

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Lab products found in correlation

Total akt, by Cell Signaling Technology (1 mentions) P akt, by Cell Signaling Technology (1 mentions) Ip lysis buffer, by Thermo Fisher Scientific (1 mentions) Proteome profiler antibody array, by R&D Systems (1 mentions) Protein g beads, by Cytiva (1 mentions) Phospho ampk, by Cell Signaling Technology (1 mentions) Ecl prime, by Cytiva (1 mentions) Complete protease inhibitor cocktail, by Roche (1 mentions) Pp2ac, by Merck Group (1 mentions) Anti striatin, by BD (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Eclipse ti, by Nikon (1 mentions) Hanks balanced salt solution (hbss), by Thermo Fisher Scientific (1 mentions) Anti cd49f fitc, by STEMCELL (1 mentions) Easysep biotin selection kit, by STEMCELL (1 mentions) Mouse mammary cell enrichment kit, by STEMCELL (1 mentions) Anti sca1 apc, by Thermo Fisher Scientific (1 mentions)

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Anti-ERα (43 citations)

2 protocols using anti erα mc20

Coimmunoprecipitation and Western Blot Analyses

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Tissue proteins were extracted in IP Lysis Buffer (Thermo Fisher Scientific) mixed with cOmplete Protease Inhibitor Cocktail (Roche), and lysates were incubated overnight at 4°C with 5 μg anti-ERα (MC20; Santa Cruz Biotechnology) or anti-striatin (BD Bioscience) antibody. Next, the lysates were incubated with protein G beads (Amersham Biosciences) for 2 h at 4°C, and the pellets obtained after centrifugation were washed five times and analyzed by immunoblotting. Proteins were resolved by dodecyl sulfate-PAGE, transferred to polyvinylidene fluoride membranes, and probed with the appropriate primary antibodies, including AMPK, GAPDH (Santa Cruz Biotechnology), PP2Ac (Millipore), phospho (p)-AMPK, p-Akt, total Akt (Cell Signaling Technology), and α-tubulin (EMD). Membranes were then incubated with the appropriate secondary antibodies and developed using ECL Prime (Amersham Biosciences). The Proteome Profiler Antibody Array (R&D Systems) was used for phospho-kinase analysis, according to the manufacturer’s instructions.

Ueda K., Takimoto E., Lu Q., Liu P., Fukuma N., Adachi Y., Suzuki R., Chou S., Baur W., Aronovitz M.J., Greenberg A.S., Komuro I, & Karas R.H. (2018). Membrane-Initiated Estrogen Receptor Signaling Mediates Metabolic Homeostasis via Central Activation of Protein Phosphatase 2A. Diabetes, 67(8), 1524-1537.

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Enrichment and Analysis of Mammary Stem/Progenitor Cells

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CD45⁺/TER119⁺, and CD31⁺ cells were removed from dissociated cells using the EasySep biotin selection kit (StemCell Technologies, Vancouver, BC, Canada) according to the manufacturer's instructions. Cells were resuspended in HBSS (Invitrogen) with 2% FBS and incubated with anti-CD24-PE (10 μl/test, Mouse mammary cell enrichment kit, StemCell Technologies), anti-CD49f- FITC (10 μl/test, Mouse mammary cell enrichment kit, StemCell Technologies) or anti-Sca1-APC (0.06 μg/test, eBioscience, San Diego, CA, USA) for 30 min on ice, followed by washing and resuspending in HBSS supplemented with 2% FBS. Analysis and sorting were performed by using a FACSVantage TurboSort SE (BD Biosciences). The data were analyzed using BD FACS Sortware sorter software. For analysis of ERα expression in Sca1⁺CD24^highCD49f⁺ and Sca1⁻CD24^highCD49f⁺ luminal cell populations, sorted cells from Tip30^−/− virgin mice (n=6) were collected and allowed to adhere to a collagen coated 96-well plate before fixation and then analyzed by immunocytochemistry with anti- ERα (MC-20, 1 : 50; Santa Cruz Biotechnology) and inverted microscopy (Nikon Eclipse Ti, Melville, NY, USA).

Chen F., Li A., Gao S., Hollern D., Williams M., Liu F., VanSickle E.A., Andrechek E., Zhang C., Yang C., Luo R, & Xiao H. (2014). Tip30 controls differentiation of murine mammary luminal progenitor to estrogen receptor-positive luminal cell through regulating FoxA1 expression. Cell Death & Disease, 5(5), e1242-.

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