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Transam nrf2 activity kit

Manufactured by Active Motif

The TransAM Nrf2 activity kit is a laboratory equipment product that measures the activity of the transcription factor Nrf2. Nrf2 is a key regulator of the cellular response to oxidative stress, and its activity is important in various biological processes. The kit provides the necessary reagents and protocols to perform this measurement in a quantitative manner.

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2 protocols using transam nrf2 activity kit

1

Quantifying Nuclear Nrf2 Activity

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Cytoplasm and nucleus proteins extracted by optimized Nuclear Extract kit (Active Motif, CA). Nuclear Nrf2 activity, measuring the ARE oligonucleotide DNA binding activity of the Nrf2, was quantified using the TransAM Nrf2 activity kit (Active Motif, CA), according to the manufacturer’s instructions.
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2

Quantifying Nrf2 Nuclear Activity

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Isolated Retina/RPE were lysed in T-PER Tissue Protein Extraction Reagent (Thermo Scientific) supplemented with protein and phosphatase inhibitors (Sigma-Aldrich). Nuclear Nrf2 activity, measuring the ARE oligonucleotide DNA binding activity of the Nrf2, was quantified in 5μg of total lysate using the TransAM Nrf2 activity kit (Active Motif, CA), according to the manufacturer’s instructions. The final A450 was read on a SpectraMax 384 Plus Multi-Mode Microplate Reader supplied with SoftMax® Pro Data Acquisition & Analysis Software (Molecular Devices).
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