Excess 1X PBS-T was removed and 35 μl of primary antibody (rat anti-SDC-3 antibody, 1:400) were added. Samples were incubated in a humid chamber for 6 hrs to overnight. Slides were washed 3 times 10 min with 1X PBS-T at room temperature and then incubated in secondary antibody (Alexa-Fluor-647 goat anti-rat antibody (Invitrogen), 1:250) for 6 hrs to overnight. Slides were then washed 3 times 10 min with 1X PBS-T at room temperature and then mounted.
Alexa fluor 647 goat anti rat antibody
Manufactured by Thermo Fisher Scientific
The Alexa-Fluor-647 goat anti-rat antibody is a fluorescently labeled secondary antibody used for detecting and visualizing rat primary antibodies in various immunoassays and imaging applications. The antibody is conjugated with the Alexa Fluor 647 dye, which has a red fluorescent emission spectrum.
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Lab products found in correlation
2 protocols using alexa fluor 647 goat anti rat antibody
1
Immunofluorescent Staining of SDC-3
2
Immunofluorescent Staining of SDC-3
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