Isotype mouse igg1 fitc

Manufactured by BioLegend

Sourced in United States

Isotype mouse IgG1–FITC is a fluorescently labeled immunoglobulin G1 (IgG1) antibody derived from mouse. It serves as an isotype control for flow cytometry and other immunoassays.

Automatically generated - may contain errors

Lab products found in correlation

Percp cy5, by BD (1 mentions) Cd80 pe, by BioLegend (1 mentions) Cd86 apc, by BioLegend (1 mentions) Facscalibur cytometer, by BD (1 mentions) Hla dr, by BD (1 mentions) Tumor necrosis factor (tnf), by R&D Systems (1 mentions) Fitc anti human cd14, by BioLegend (1 mentions) Anti nfatc1 antibody 7a6, by Thermo Fisher Scientific (1 mentions) Soluble rankl, by R&D Systems (1 mentions) Il 23, by R&D Systems (1 mentions)

Spelling variants of this product

Mouse IgG1 (41 citations) FITC mouse IgG1 (20 citations) FITC-IgG1 (9 citations) FITC mouse IgG1 κ isotype control (6 citations) FITC Mouse IgG1 κ Isotype Ctrl antibody (3 citations) Isotype control mouse IgG1 FITC (MOPC-21, (2 citations)

2 protocols using isotype mouse igg1 fitc

Phenotypic Analysis of Dendritic Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The CD14 (PerCP-Cy 5.5, BD Biosciences, United States), HLA-DR (APC, BD Biosciences, United States), and isotype mouse IgG2a-PE (PE, BioLegend, United States) were added in the samples of imDCs. Moreover, CD86-APC, CD80-PE, and isotype mouse IgG1–FITC (BioLegend, United States) were added in the samples of mDCs. The cells were then suspended with precooled PBS, counted under a microscope, and centrifuged at 1000 g for 5 min. Data were acquired using a FACSCalibur cytometer (BD Biosciences, United States) and the ratios of CD14⁺, HLA-DR⁺, CD80⁺, and CD86⁺ DCs were determined.

Zhou Y., Chen X., Zheng Y., Shen R., Sun S., Yang F., Min J., Bao L., Zhang Y., Zhao X., Wang J, & Wang Q. (2021). Long Non-coding RNAs and mRNAs Expression Profiles of Monocyte-Derived Dendritic Cells From PBMCs in AR. Frontiers in Cell and Developmental Biology, 9, 636477.

+ Open protocol

+ Expand

Osteoclastogenesis Signaling Pathway Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Soluble RANKL, OPG, IL-23, TNF, RANKL ELISA and anti-MDL-1/CLEC5A antibody
(283834) were purchased from R&D Systems (USA). Anti-phospho-SYK (Y525/526, Y323,
Y352), anti-PLCγ2 (polyclonal), and anti-phospho-PLCγ2 (Y759) antibodies
were purchased from Cell Signaling (USA). Anti-SYK antibody (SYK-01) was purchased from
Abcam (UK). Anti-GAPDH, (6C5) anti-SRC (GD11), anti-phospho-SRC (Y418),
anti-phosphotyrosine (4G10) antibodies were purchased from Millipore (USA). Anti-human
CD16 PE-cy7 (clone CB16), isotype Mouse IgG1κ and anti-human CD16 PE-cy5 (clone
3G8) and isotype mouse IgG1 PE-cy5 were from Ebioscience USA and Biolegend USA
respectively. Anti-human CD14 FITC and isotype Mouse IgG1 FITC were from Biolegend, USA.
Anti-human DAP-12 PE and isotype control Rat IgG1 PE were purchased from Beckman Coulter
and Ebioscience, USA. Anti-NFATc1 antibody (7A6) was purchased from Thermo Scientific
(USA). Anti-DAP12 antibody (406288) was purchased from Exalpaha (USA). Anti-IL-23R (H-300)
was purchased from Santa Cruz (USA).

SHIN H.S., SARIN R., DIXIT N., WU J., GERSHWIN M.E., BOWMAN E.P, & ADAMOPOULOS I.E. (2014). Crosstalk among interleukin 23 and DNAX activating protein 12-dependent pathways promotes osteoclastogenesis. Journal of immunology (Baltimore, Md. : 1950), 194(1), 316-324.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!