One hundred μl DEPC water dissolved 0.5 μM miR-34a-5p antagomir was introduced by tail vein injection immediately after TAI. DEPC water with negative control was administrated as control. Morris water maze tests were performed following the aforementioned protocol. miR-34a-5p antagomir and negative control were synthesized by RiboBio (Guangzhou, China). The tail vein injection performed at the next day after TAI exposure, n = 12 per group.
Mir 34a 5p antagomir
Manufactured by RiboBio
Sourced in China
MiR-34a-5p antagomir is a synthetic oligonucleotide designed to inhibit the activity of miR-34a-5p, a microRNA involved in various cellular processes. This product can be used in research applications to study the biological functions of miR-34a-5p.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mir 34a 5p antagomir
1
Mitigating TAI-Induced Cognitive Deficits
2
miR-34a and DLL1 Regulation Pathway
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The Homo sapien miR-34a-5pmimics, miR-34a-5p antagomiR, DLL1 siRNA and their corresponding scramble sequences (negative control, NC) and the transfection kit were supplied by Guangzhou Ribobio, China. The mammalian expression constructs for DLL1 (EX-Y3540-M98-5) with GFP tag were supplied by Guangzhou Fulengen, China. Transfection of the ribonucleic acid reagents or plasmids mentioned in this paper and the reporter plasmids in Cignal Finder™ Pathway Reporter Arrays (SABiosciences, USA) has been described previously25 (link). The sequences used in this study are as follows:
si-DLL1: 5′-CCAUAAGCCCUGCAAGAAU dTdT-3′ 3′-dTdT GGUAUUCGGGACGUUCUUA-5′ hsa-miR-34a-5p antagomiR: 5′ACAACCAGCUAAGACACUGCCA 3′ mimics: sense 5′UGGCAGUGUCUUAGCUGGUUGU 3′ antisense 5′ACAACCAGCUAAGACACUGCCA 3′
si-DLL1: 5′-CCAUAAGCCCUGCAAGAAU dTdT-3′ 3′-dTdT GGUAUUCGGGACGUUCUUA-5′ hsa-miR-34a-5p antagomiR: 5′ACAACCAGCUAAGACACUGCCA 3′ mimics: sense 5′UGGCAGUGUCUUAGCUGGUUGU 3′ antisense 5′ACAACCAGCUAAGACACUGCCA 3′
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