Dulbecco s modified eagle s medium f12 dmem f12

Dulbecco’s
Modified Eagle’s Medium F-12 (DMEM F-12), antibiotic and antimycotic
solution, trypsin-EDTA solution (0.25%), l-histidine, 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyl
tetrazolium bromide (MTT), and Hank’s Balanced Salt Solution
(HBSS) were purchased from Sigma-Aldrich (Sigma St. Louis, MO, USA).
Fetal bovine serum (FBS) was purchased from Gibco Life Technologies,
USA. Polysorbate 80, hematoxylin, eosin, xylene, chloroform, and paraffin
were purchased from HiMedia Laboratories, Mumbai, India. Other chemicals
and reagents used were of analytical grade. Sandalwood oil was procured
from Shree Krishna Medicose, Rajnandgaon, India.

Norfluoxetine (NFLX) was purchased from LGC Standards (Germany). Resazurin, 17β-estradiol (E₂), EDTA, tricine, dithiothreitol (DTT), ATP and Dulbecco’s Modified Eagle’s Medium F-12 (DMEM F-12) were obtained from Sigma Aldrich (Steinheim, Germany). Tris and 1,2-diaminocyclohexane-N,N,N′,N′-tetraacetic acid (CDTA) were from Fluka (Buchs, Switzerland) and luciferin was from Roth (Karlsruhe, Germany). (MgCO₃)₄Mg(OH)₂.5H₂O was purchased from Acros Organics (Geel, Belgium). Dulbecco’s Phosphate Buffered Saline (PBS), trypsin, RPMI1640 + GlutaMAX culture medium and charcoal stripped Fetal Bovine Serum (FBS) and were purchased from Gibco (Paisley, UK). Fetal Bovine Serum (FBS) was from Sigma (Steinheim, Germany).

Rat UII was acquired from Phoenix Pharmaceuticals Inc. (Belmont, CA, USA). A rat ALD enzyme-linked immunosorbent assay (ELISA) kit was obtained from Sangon Biotech (Shanghai, China). Fetal bovine serum (FBS) was acquired from HyClone (Logan, UT, USA). The ALD antibody from Novus Biologicals (Littleton, CO, USA), the ALD-R antibody from Enzo Life Sciences (Lausen, Switzerland). The calcineurin inhibitor cyclosporin A (CSA), the Ca²⁺ channel blocker nicardipine and Dulbecco's modified Eagle's medium/F12 (DMEM/F12) were purchased from Sigma-Aldrich; Merck KGaA (St. Louis, MO, USA). The signal transduction blockers PD98059 (for mitogen-activated protein kinase), Y-27632 (for Rho-associated protein kinase) and H-7 (for protein kinase C) were acquired from Calbiochem (Darmstadt, Germany); High Capacity cDNA Reverse Transcription kits and SYBR Select Master Mix were purchased from Applied Biosystems (Foster City, CA, USA); and RIPA lysis buffer and TRIzol reagent were acquired from Invitrogen (Carlsbad, CA, USA). PCR primers were designed and synthesized by Sangon Biotech (Shanghai, China), and their sequences are shown in Table I. All other chemicals and reagents were of analytical grade.

Michigan Cancer Foundation-7 (MCF-7) cell line and Normal Human Dermal Fibroblasts (NHDF) were obtained from ATCC (Middlesex, UK) and Promocell (Heidelberg, Germany), respectively. 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) was bought from Promega (Madison, WI, USA). Fetal Bovine Serum (FBS) was acquired from Biochrom AG (Berlin, Germany). 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) was purchased from Merck (Darmstadt, Germany). Dimethyl sulfoxide (DMSO) and methanol were acquired from Fisher Scientific (Oeiras, Portugal). Cell imaging plates were obtained from Ibidi GmbH (Munich, Germany). Cell culture plates and T-flasks were purchased from Thermo Fisher Scientific (Porto, Portugal). GO was obtained from NanoPoz (Umultowska Poznan, Wielkopolska). Branched PEI (M_n 1250 Da), SBMA, Dulbecco's Modified Eagle's Medium-F12 (DMEM-F12), IR780 iodide, N-hydroxysuccinimide (NHS), paraformaldehyde, PMAO (average M_n 30 000–50 000 Da) and trypsin were bought from Sigma-Aldrich (Sintra, Portugal). Calcein-AM, Hoechst 33342® and Propidium Iodide (PI) were obtained from Thermo Fisher Scientific (Porto, Portugal). Water used in all experiments was double deionized (0.22 μm filtered, 18.2 MΩ cm).