Genomic DNA was extracted using the Gentra Puregen DNA Extraction Kit (Qiagen) according to the manufacturer’s instructions. Each DNA sample was evaluated on a Nanodrop (Thermo Scientific) for concentration and purity, and integrity of DNA was determined by resolving 20 ng of DNA on a 1% agarose gel20 (link).
Gentra puregen dna extraction kit
Manufactured by Qiagen
Sourced in United States
The Gentra Puregen DNA Extraction Kit is a laboratory equipment product designed for the efficient extraction and purification of DNA from a variety of sample types. It provides a reliable and consistent method for isolating genomic DNA.
Automatically generated - may contain errors
Lab products found in correlation
Nanodrop, by Thermo Fisher Scientific (1 mentions)
Stratagene mx3005p, by Agilent Technologies (1 mentions)
Nanodrop spectrophotometer, by Thermo Fisher Scientific (1 mentions)
Goscript reverse transcription system, by Promega (1 mentions)
Thermocycler, by Bio-Rad (1 mentions)
Rneasy plant mini kit, by Qiagen (1 mentions)
Hybond n membrane, by GE Healthcare (1 mentions)
Anti dig antibody, by Roche (1 mentions)
Hinfi and rsai, by New England Biolabs (1 mentions)
Cdp star, by Roche (1 mentions)
4 protocols using gentra puregen dna extraction kit
1
Genomic DNA Extraction and Evaluation
2
DNA Extraction and Quality Assessment
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DNA was extracted using the Gentra Puregen DNA Extraction Kit (Qiagen, Valencia, CA, USA) and integrity was determined by resolving 20 ng of DNA on a 1% agarose. All samples passed the integrity test.
3
Rice Gene Expression Analysis
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Rice stems and leaves of post-tillering phase were collected and fine-ground in liquid nitrogen. Total DNA was extracted by using Gentra Puregen DNA Extraction Kit (Qiagen). The concentration and integrity of genome DNA was measured with NanoDrop™ Spectrophotometer (Thermo Fisher). Total RNA was extracted by using RNeasy Plant Mini Kit (Qiagen). Primers were designed by using NCBI on-line tool Primer-BLAST [25] (Supplementary Table 1) and tested by using Thermocycler (Bio-rad) (Supplementary Fig. 1). First-strand cDNA were synthesized by using GoScript™ Reverse Transcription System (Promega). Quantitative PCR were performed in StrataGene Mx3005P (Agilent).
The statistical analysis of the gene expression was performed by using SAS software package (SAS INC). Duncan multiple range test and T test were calculated with significant level (P = 0.05), and extreme significant level (P = 0.01).
The statistical analysis of the gene expression was performed by using SAS software package (SAS INC). Duncan multiple range test and T test were calculated with significant level (P = 0.05), and extreme significant level (P = 0.01).
4
Telomere Length Quantification Protocol
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Genomic DNA was extracted by Gentra Puregen DNA Extraction Kit (Qiagen). DNA (3 μg) was digested with HinF I and Rsa I (NEB) overnight and separated on a 0.85% agarose gel at 2 V/cm for 16 hr. DNA was transferred from gel to Hybond‐N+ membrane (GE) and fixed by UV crosslinking. The membrane was hybridized with DIG‐labeled telomere probe overnight at 42 °C, followed by washing with 2× SSC, 0.1% SDS buffer for 15 min, 0.5× SSC, and 0.1% SDS buffer at 55 °C for 15 min twice. The membrane was incubated with 1× DIG blocking solution and then with anti‐DIG antibody (Roche) for 30 min. Telomere signals were detected by incubating with CDP‐star (Roche) for 5 min. The average telomere length was quantified using telotool software.
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