Sh fus 1 2

Specific shRNAs against LINC01106 (sh/LINC01106#1/2), FUS (sh/FUS#1/2), Gli1 (sh/Gli1#1/2), Gli2 (sh/Gli2#1/2), and their relative negative control (sh/ctrl), were obtained from Genechem (Shanghai, China), together with pcDNA3.1/Gli4, pcDNA3.1/FUS, pcDNA3.1/Gli1, pcDNA3.1/Gli2, and the pcDNA3.1 empty vector (negative control). MiRNA mimics and NC mimics, miR-449b-5p inhibitor and NC inhibitor were all synthesized by GenePharma (Shanghai, China). All above plasmids were constructed into the pLKO.1 vector (Addgene #10878), followed by lentiviral particle preparation as previously described^{26 (link)}. Thereafter, lentiviral particles containing above plasmids were separately transfected into LoVo or SW1116 cells for 48 h by Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA). Finally, stably transfected cells were screened out via 2 μg/ml of puromycin treatment for additional 48 h.

Specific shRNAs against LDHB (sh-LDHB#1/2) or FUS (sh-FUS#1/2) and NC-shRNAs (sh-NC), along with pcDNA3.1/FUS (OE-FUS), pcDNA3.1/LDHB (OE-LDHB) and the empty vector (OE-NC), were acquired from Genechem (Shanghai, China). Moreover, miR-141-3p mimics and NC mimics were obtained from GenePharma (Shanghai, China). Plasmids were separately transfected into Saos-2 or HOS cells through Lipofectamine 3000 (Invitrogen, Carlsbad, CA, U.S.A.).