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Rt2 custom profiler pcr arrays

Manufactured by Qiagen
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Sourced in Germany

The RT2 Custom Profiler PCR Arrays are a pre-designed and pre-validated set of primer pairs for quantifying the expression of a customizable panel of genes. The arrays enable efficient and accurate real-time PCR analysis of gene expression profiles.

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Lab products found in correlation

Pico chip 5067 1511, by Agilent Technologies (2 mentions) Trizol reagent, by Thermo Fisher Scientific (2 mentions) Lightcycler 480, by Roche (1 mentions) Rneasy mini kit, by Qiagen (1 mentions)

Spelling variants of this product

RT2 Profiler PCR Array (622 citations) Custom RT2 Profiler PCR Array (35 citations) RT2 Profiler Arrays (7 citations) Custom PCR arrays (5 citations) Custom RT2 PCR Arrays (4 citations) RT2 PCR arrays (3 citations) Custom RT2 Profiler Array (2 citations) Profiler PCR arrays (2 citations) Custom RT2 Profiler PCR Arrays and Primer Assays (2 citations)

4 protocols using rt2 custom profiler pcr arrays

1

RT2 Custom Profiler PCR Arrays Validation

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The selected genes were validated using RT2 Custom Profiler PCR Arrays (Supplementary Table S2; Qiagen, Hilden, Germany), according to the manufacturer’s instructions, using a LightCycler® 480 (Roche Life Science, Mannheim, Germany). These PCR arrays were also performed on RNA samples from HUVEC and HRMVEC cultured for 5 days in ¼ ECGM-2 NG/HG (three and one biological replicate, respectively).
Kremer H., Gebauer J., Elvers-Hornung S., Uhlig S., Hammes H.P., Beltramo E., Steeb L., Harmsen M.C., Sticht C., Klueter H., Bieback K, & Fiori A. (2020). Pro-angiogenic Activity Discriminates Human Adipose-Derived Stromal Cells From Retinal Pericytes: Considerations for Cell-Based Therapy of Diabetic Retinopathy. Frontiers in Cell and Developmental Biology, 8, 387.
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2

Axonal Guidance Molecules in Adipose Tissue

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1 mg of adipose tissue from either lean or obese animals was homogenized in TRIzol reagent (Invitrogen) using the Bullet BlenderTM (New England Bio Group) and total RNA isolated as we described33 (link). RNA quality was verified by pico chip (5067-1511; Agilent technologies). Adipose RNA (1 µg) was reverse transcribed and quantitative RT-PCR analysis of the 4 families of the axonal guidance molecules was performed using RT2 Custom Profiler PCR Arrays (Qiagen) according to manufacturer’s protocol. Data analysis was performed using the manufacturer’s integrated web-based software package of the PCR Array System using ΔΔCt based fold- change calculations.
Ramkhelawon B., Hennessy E.J., Ménager M., Ray T.D., Sheedy F.J., Hutchison S., Wanschel A., Oldebeken S., Geoffrion M., Spiro W., Miller G., McPherson R., Rayner K.J, & Moore K.J. (2014). Netrin-1 promotes adipose tissue macrophage accumulation and insulin resistance in obesity. Nature medicine, 20(4), 377-384.
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3

Axonal Guidance Molecules in Adipose Tissue

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1 mg of adipose tissue from either lean or obese animals was homogenized in TRIzol reagent (Invitrogen) using the Bullet BlenderTM (New England Bio Group) and total RNA isolated as we described33 (link). RNA quality was verified by pico chip (5067-1511; Agilent technologies). Adipose RNA (1 µg) was reverse transcribed and quantitative RT-PCR analysis of the 4 families of the axonal guidance molecules was performed using RT2 Custom Profiler PCR Arrays (Qiagen) according to manufacturer’s protocol. Data analysis was performed using the manufacturer’s integrated web-based software package of the PCR Array System using ΔΔCt based fold- change calculations.
Ramkhelawon B., Hennessy E.J., Ménager M., Ray T.D., Sheedy F.J., Hutchison S., Wanschel A., Oldebeken S., Geoffrion M., Spiro W., Miller G., McPherson R., Rayner K.J, & Moore K.J. (2014). Netrin-1 promotes adipose tissue macrophage accumulation and insulin resistance in obesity. Nature medicine, 20(4), 377-384.
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4

Quantifying Netrin-1 Family Expression

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Total RNA was extracted using RNeasy Mini Kit (Qiagen, Invitrogen) including sample homogenization with QIAshredder columns from both undifferentiated and osteoclast-derived RAW264.7 cells. RNA quality was verified by Pico Chips (5067-1511; Agilent Technologies, Santa Clara, CA, USA). RNA (1 µg) was reverse-transcribed, and quantitative RT-PCR analysis of the Netrin-1 guidance cue family members was performed using RT2 Custom Profiler PCR Arrays (Qiagen), according to the manufacturer’s protocol. Data analysis was performed using the manufacturer’s integrated web-based software package of the PCR Array System using ΔΔCt-based fold change calculations.
Mediero A., Ramkhelawon B., Perez-Aso M., Moore K.J, & Cronstein B.N. (2015). Netrin-1 Is a Critical Autocrine/Paracrine Factor for Osteoclast Differentiation. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 30(5), 837-854.
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