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Bactec plus aerobic f and anaerobic f bottles

Manufactured by BD
Sourced in United States

The Bactec-plus aerobic/F and anaerobic/F bottles are laboratory equipment used for the detection and cultivation of microorganisms from clinical samples. These bottles provide a controlled environment for the growth of aerobic and anaerobic bacteria, respectively.

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2 protocols using bactec plus aerobic f and anaerobic f bottles

1

Antibiotic Resistance Profiling of Blood Isolates

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Patients provided 20-mL blood specimens that were divided into 10-mL aliquots in Bactec-plus aerobic/F and anaerobic/F bottles (BD Diagnostic Systems, Franklin Lakes, NJ, USA) and immediately subjected to microbiological testing. The specimens were incubated in a Bactec Fx instrument (BD Diagnostic Systems) and bacterial organisms isolated from positive blood cultures were examined using conventional overnight identification/sensitivity panels with a MicroScan WalkAway system (Siemens, West Sacramento, CA, USA). Tested antibiotic classes included ampicillin/sulbactam, aztreonam, cephalosporins (three third-generation and one fourth-generation), aminoglycosides (gentamicin and tobramycin), quinolones (ciprofloxacin and levofloxacin), carbapenems (imipenem and meropenem), tetracycline, and trimethoprim/sulfamethoxazole. Isolates were considered carbapenem-resistant if they were resistant to imipenem or meropenem.
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2

Blood Culture Analysis at Kandgond Hospital

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Two hundred and forty-nine positive blood cultures were analyzed from patients admitted to Kandgond Sacred-Heart Hospital in Seoul from March 2014 to June 2015. Duplicated blood cultures from the same patients were excluded. Gram-positive bacteria were cultured from 99 samples and Gram-negative bacteria from 150 samples. Blood specimens were inoculated into Bactec-plus aerobic/F and anaerobic/F bottles (BD Diagnostic Systems) and incubated in the Bactec Fx instrument (BD Diagnostic Systems).
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