Srebp 2 antibody

Manufactured by Abcam

Sourced in United States

The SREBP-2 antibody is a research-use only product that specifically recognizes the SREBP-2 protein. SREBP-2 is a transcription factor that plays a key role in regulating the expression of genes involved in cholesterol biosynthesis and uptake.

Automatically generated - may contain errors

Lab products found in correlation

Varespladib, by Selleck Chemicals (2 mentions) Pngase f, by Promega (2 mentions) Random primer, by Thermo Fisher Scientific (1 mentions) Enhanced chemiluminescence western blotting detection reagent, by GE Healthcare (1 mentions) Superscript 2, by Thermo Fisher Scientific (1 mentions) Pierce bicinchoninic acid protein assay kit, by Thermo Fisher Scientific (1 mentions) Horseradish peroxidase conjugated antirabbit antibodies, by Bio-Rad (1 mentions) Imagequant 5, by GE Healthcare (1 mentions) Recombinant human pro mmp 2, by Merck Group (1 mentions) Mcp 3, by R&D Systems (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Oil red o stain, by Merck Group (1 mentions) Alkaline phosphatase, by Merck Group (1 mentions) Cholesterol, by Merck Group (1 mentions) Protein assay, by Bio-Rad (1 mentions) Enhanced chemiluminescence immunoblotting detection reagent, by GE Healthcare (1 mentions) Hrp conjugated antirabbit antibodies, by Bio-Rad (1 mentions) Protein assay kit, by Bio-Rad (1 mentions) β actin antibody, by Cell Signaling Technology (1 mentions)

3 protocols using srebp 2 antibody

Oil Red O Staining for Lipid Metabolism

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Oil Red O stain, alkaline phosphatase, and cholesterol were from Sigma‐Aldrich. Dulbecco minimum essential medium, antibodies against liver X receptor (LXR), TaqMan quantitative reverse transcription–polymerase chain reaction (qRT‐PCR) primers, TRIzol reagent, random primers, Superscript II, and penicillin/streptomycin were from Life Technologies. SREBP‐2 antibody was from Abcam. The “high carb” TD.88122 mouse diet (74% calories from carbohydrates) was from Harlan Laboratories. Recombinant human pro–MMP‐2 was from EMD Millipore. Collagen‐coated cell dishes were from Greiner Bio‐One. Varespladib was from Selleck Chemicals. PNGase F was from Promega. Enhanced chemiluminescence western blotting detection reagent was from GE Healthcare. Horseradish peroxidase–conjugated antirabbit antibodies and Bio‐Rad Protein Assay were from Bio‐Rad. The Pierce bicinchoninic acid protein assay kit was from Thermo Scientific. MCP‐3, neutralizing MCP‐3 antibody, and control isotype‐matched IgG₁ were from R&D Systems, Inc. Densitometry was performed using ImageQuant 5.1 (Molecular Dynamics).

Hernandez‐Anzaldo S., Berry E., Brglez V., Leung D., Yun T.J., Lee J.S., Filep J.G., Kassiri Z., Cheong C., Lambeau G., Lehner R, & Fernandez‐Patron C. (2015). Identification of a Novel Heart–Liver Axis: Matrix Metalloproteinase‐2 Negatively Regulates Cardiac Secreted Phospholipase A2 to Modulate Lipid Metabolism and Inflammation in the Liver. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 4(11), e002553.

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Quantifying SREBP-2 Pathway Activation

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Sterol regulatory element binding protein 2 (SREBP‐2) antibody was purchased from Abcam. Varespladib was from Selleck Chemicals. PNGase F was from Promega. Enhanced chemiluminescence immunoblotting detection reagent was from GE Healthcare. HRP‐conjugated antirabbit antibodies and the Bio‐Rad Protein Assay kit were from Bio‐Rad Laboratories.

Hernandez‐Anzaldo S., Brglez V., Hemmeryckx B., Leung D., Filep J.G., Vance J.E., Vance D.E., Kassiri Z., Lijnen R.H., Lambeau G, & Fernandez‐Patron C. (2016). Novel Role for Matrix Metalloproteinase 9 in Modulation of Cholesterol Metabolism. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 5(10), e004228.

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Antibody Staining and Analysis Protocol

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SOX10 antibody (ab155279; Abcam, Cambridge, MA, USA; 1:200), P75NTR antibody (Abcam, ab52987; 1:50), S100 antibody (Abcam, ab52642; 1:100), MPZ antibody (#57518; Cell Signaling Technology, Danvers, MA, USA; 1:100), and Cy5 AffiniPure Goat Anti-Rabbit IgG (H+L) (111-175-144; Jackson ImmunoResearch Laboratories, West Grove, PA, USA; 1:200) were used for cellular immunofluorescence. AKR1C2 antibody (PA5-36572; Invitrogen, Carlsbad, CA, USA; 1:500), AKR1C3 antibody (Abcam, ab84327; 1:500), SCD antibody (Cell Signaling Technology, #2794; 1:1000), SREBP1 antibody (2A4) (sc-13551; Santa Cruz Biotechnology, Santa Cruz, CA, USA; 1:500), SREBP2 antibody (Abcam, ab30682; 1:500), ASPM antibody (Invitrogen, PA5-99790; 1:500), TOP2A antibody (Cell Signaling Technology, #12286; 1:1000), β-ACTIN antibody (Cell Signaling Technology, #3700; 1:1000), α-TUBULIN antibody (Cell Signaling Technology, #2125; 1:1000), GAPDH antibody (60004-1-IG; Proteintech, Rosemont, IL, USA; 1:20000), horseradish peroxidase-labeled goat anti-mouse IgG (H+L) (Affinity Purified) (ZB-5305; ZSGB-BIO Co., Ltd., Beijing, China; 1:4000), and horseradish-peroxidase-labeled goat anti-rabbit IgG (H+L) (Affinity Purified) (ZSGB-BIO, ZB-5301; 1:4000) were used for western blotting. Alexa Fluor 488 anti-human Ki-67 antibody (350508; Biolegend, San Diego, CA, USA) was used for flow cytometry.

Ma S., Mi Z., Wang Z., Sun L., Liu T., Shi P., Wang C., Xue X., Chen W., Wang Z., Yu Y., Zhang Y., Bao F., Wang N., Wang H., Xia Q., Liu H., Sun Y, & Zhang F. (2023). Single-cell sequencing analysis reveals development and differentiation trajectory of Schwann cells manipulated by M. leprae. PLOS Neglected Tropical Diseases, 17(7), e0011477.

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