Mice were treated with Tamoxifen (2 mg/kg/day) using a regimen that we have previously shown synergistically enhanced loading-related bone gain [28 (link)]. At 16 weeks of age (day 1), 16 virgin female C57BL/6 mice were OVX. Ten days after surgery (day 11), the OVX mice were randomly subdivided into two groups (n = 8) and received either vehicle (peanut oil, 5 ml/kg; Sigma) or tamoxifen citrate (Tocris Cookson Inc., Ellisville, MO) by s.c. injection on days 11, 13, 15, 18, and 21 and were then sacrificed on day 25. Tibias were immediately snap frozen in liquid nitrogen and later used for qRT-PCR.
Tamoxifen citrate
Manufactured by Bio-Techne
Sourced in United Kingdom
Tamoxifen citrate is a non-steroidal anti-estrogen compound. It is a selective estrogen receptor modulator (SERM) that is used in various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Myriocin, by Cayman Chemical (2 mentions)
Mpp dihydrochloride, by Bio-Techne (2 mentions)
Pkcζ ps, by Bio-Techne (2 mentions)
Dl pdmp, by Bio-Techne (2 mentions)
G 6983, by Bio-Techne (2 mentions)
G 6976, by Bio-Techne (2 mentions)
Peanut oil, by Merck Group (1 mentions)
Spectrum two ft ir spectrometer, by PerkinElmer (1 mentions)
Olaparib, by MedChemExpress (1 mentions)
4 protocols using tamoxifen citrate
1
Ovariectomized Mice Treated with Tamoxifen
2
Evaluation of Novel Signaling Modulators
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Tamoxifen citrate, ceramide, MPP dihydrochloride, G-1, Gö 6983, Gö 6976, PKCζ-PS and DL-PDMP were all obtained from Tocris Bioscience (Bristol, UK). Myriocin was obtained from Cayman Chemical (Ann Arbor, MI). Unless otherwise noted, all other chemicals were obtained from Sigma Aldrich.
3
Evaluation of Novel Signaling Modulators
4
Synthesis and Characterization of Novel Compounds
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Olaparib and Ceralasertib
were purchased from MedChemExpress, Berzosertib was purchased from
Abcam and Tamoxifen citrate was purchased from Tocris. All other chemicals
were purchased from SigmaAldrich and ThermoFisher Scientific. All
commercial reagents were used without purification unless otherwise
specified. 1H and 13C NMR spectra were obtained
using a Bruker Advance III 500 MHz Nuclear Magnetic Resonance Spectrometer.
HRMS (high-resolution mass spectroscopy) samples were analyzed at
the EPSRC UK National Mass Spectrometry Facility at Swansea University
using a ThermoScientific LTQ Orbitrap XL 1 Mass Spectrometer. Fourier
transform infrared spectra were run on a Perkin Elmer FT-IR Spectrometer
Spectrum TWO. Elemental analysis was performed by the Elemental Analysis
Service at London Metropolitan University. Analytical HPLC was carried
out on the Agilent system equipped with a Waters XBridge C18 (130
Å, 3.5 μm, 4.6 × 150 mm) analytical column. Water
with 0.1% trifluoroacetic acid (TFA) and acetonitrile with 0.1% TFA
were used as eluents. The flow rate was 1 mL/min, and 50% A:50% B
was used for 15 min. The purity of the final compounds was ≥95%
as determined by HPLC or elemental microanalysis.
were purchased from MedChemExpress, Berzosertib was purchased from
Abcam and Tamoxifen citrate was purchased from Tocris. All other chemicals
were purchased from SigmaAldrich and ThermoFisher Scientific. All
commercial reagents were used without purification unless otherwise
specified. 1H and 13C NMR spectra were obtained
using a Bruker Advance III 500 MHz Nuclear Magnetic Resonance Spectrometer.
HRMS (high-resolution mass spectroscopy) samples were analyzed at
the EPSRC UK National Mass Spectrometry Facility at Swansea University
using a ThermoScientific LTQ Orbitrap XL 1 Mass Spectrometer. Fourier
transform infrared spectra were run on a Perkin Elmer FT-IR Spectrometer
Spectrum TWO. Elemental analysis was performed by the Elemental Analysis
Service at London Metropolitan University. Analytical HPLC was carried
out on the Agilent system equipped with a Waters XBridge C18 (130
Å, 3.5 μm, 4.6 × 150 mm) analytical column. Water
with 0.1% trifluoroacetic acid (TFA) and acetonitrile with 0.1% TFA
were used as eluents. The flow rate was 1 mL/min, and 50% A:50% B
was used for 15 min. The purity of the final compounds was ≥95%
as determined by HPLC or elemental microanalysis.
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