Mouse monoclonal antibody against e cadherin

Manufactured by BD

Mouse monoclonal antibody against E-cadherin. This antibody is designed to detect E-cadherin, a cell-cell adhesion molecule, in various applications.

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Lab products found in correlation

Rabbit monoclonal antibody against α sma, by Abcam (1 mentions) Mouse monoclonal antibodies against α tubulin, by Merck Group (1 mentions) Mouse monoclonal antibody against gapdh, by Santa Cruz Biotechnology (1 mentions) Irdye 800cw secondary antibody, by LI COR (1 mentions) Mirneasy isolation kit, by Qiagen (1 mentions) Normal donkey serum (nds), by Jackson ImmunoResearch (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (1 mentions) Antigen retrieval solution, by Vector Laboratories (1 mentions) Nupage 4 12 bis tris gel, by Thermo Fisher Scientific (1 mentions) Mouse monoclonal antibody against beta actin, by Merck Group (1 mentions) Quantinova sybr green pcr mix, by Qiagen (1 mentions) Rabbit polyclonal antibody against α sma, by Abcam (1 mentions) Irdye 680rd, by LI COR (1 mentions) P erk, by Cell Signaling Technology (1 mentions) P akt, by Cell Signaling Technology (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions) Rabbit monoclonal antibody against vimentin, by Abcam (1 mentions)

Close spelling variants of this product

E-cadherin (700 citations) E-cadherin antibody (33 citations) Mouse E-cadherin (12 citations) Antibody against E-cadherin (9 citations)

3 protocols using mouse monoclonal antibody against e cadherin

Immunohistochemical Antibody Characterization

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Rabbit monoclonal antibody against α-SMA (for histological staining) was from Abcam (Cambridge, UK). Polyclonal rabbit antibody against fibronectin was a generous gift from Dr. Deane Mosher at the University of Wisconsin-Madison. Mouse monoclonal antibodies against α-tubulin, α-SMA and vinculin were from Sigma-Aldrich. Rabbit polyclonal antibody against total fibronectin was from Abcam. Mouse monoclonal antibody against GAPDH was from Santa Cruz Biotech (Santa Cruz, CA). Rabbit polyclonal antibody against total MKL1 was from Bethyl (Montgomery, TX). Mouse monoclonal antibody against E-Cadherin was from BD Biosciences (San Jose, CA).

Bernau K., Ngam C., Torr E.E., Acton B., Kach J., Dulin N.O, & Sandbo N. (2015). Megakaryoblastic leukemia-1 is required for the development of bleomycin-induced pulmonary fibrosis. Respiratory Research, 16(1), 45.

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Quantification of Protein Expression in Cells

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The miRNeasy isolation kit and the QuantiNova SYBR Green PCR mix were purchased from Qiagen (Valencia, CA). Running buffer, transfer buffer, NuPage 4–12% Bis-Tris gels, and Invitrolon polyvinylidene fluoride (PVDF) membranes (0.45 µm) were procured from Invitrogen (Carlsbad, CA) while the blocking buffer was from Li-Cor Biosciences (Lincoln, NE). Antigen retrieval solution and 4',6-diamidino-2-phenylindole (DAPI) were from Vector Laboratories (Burlingame, CA), and the normal donkey serum was purchased from Jackson ImmunoResearch Laboratories (Westgrove, PA). The following primary antibodies were used for immunohistochemistry and western blotting: rabbit monoclonal antibody against aquaporin 5 (1:100 or 1:5,000; Abcam Inc., Cambridge, MA); mouse monoclonal antibody against E-cadherin (1:100 or 1:5,000; BD Biosciences, San Jose, CA), rabbit polyclonal antibody against α-SMA (1:100 or 1:5,000; Abcam Inc.), and mouse monoclonal antibody against beta actin (1:10,000; Sigma-Aldrich Co., St. Louis, MO). Fluorescein isothiocyanate (FITC) or tetramethylrhodamine (TRITC) conjugated secondary antibodies (1:100, Jackson ImmunoResearch Laboratories) were used for immunohistochemical staining, while IRDye 680RD or IRDye 800CW secondary antibodies (1:5,000; Li-Cor Biosciences) were used for western blotting.

Hawley D., Aluri H., Armaos H., Kim G., Kublin C, & Zoukhri D. (2016). Human postmortem lacrimal and submandibular glands stored in RNAlater are suitable for molecular, biochemical, and cell biological studies. Molecular Vision, 22, 1221-1228.

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Western Blotting and Subcellular Fractionation

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Western blotting was performed as described previously [25 (link), 36 (link)]. The antibodies included: mouse monoclonal antibodies against Anxa2, p-Anxa2 (Y23), Slug, and β-actin from Santa Cruz Biotechnology; rabbit monoclonal antibodies against EGFR, STAT3, p-STAT3 (Y705), Erk1/2, p-Erk, Akt, p-Akt, and Histone from Cell Signaling Technology; mouse monoclonal antibody against E-cadherin from BD Biosciences; and rabbit monoclonal antibody against Vimentin from Abcam. After incubation with the above primary antibodies overnight at 4°C, the membranes were washed with TBST three times then incubated with secondary antibodies in Odyssey blocking buffer (Gene Company) for 1 h at room temperature. The immunoreactive bands were determined by image scanning on an Image Station LI-COR Odyssey imaging system (Gene Company) and analyzed using the image software. β-actin was used as internal control.
Separation of membranous and cytoplasmic protein fractions from nuclear protein fractions
The formula of lysis buffer and method of subcellular fractionation referred to the instruction by Abcam and the method in the previous study [48 (link)]. The protein fractions were prepared for Western blot analysis. β-actin was used as internal control for membrane and cytoplasm fractions. Histone was used as control for nucleus protein fractions.

Wang T., Yuan J., Zhang J., Tian R., Ji W., Zhou Y., Yang Y., Song W., Zhang F, & Niu R. (2015). Anxa2 binds to STAT3 and promotes epithelial to mesenchymal transition in breast cancer cells. Oncotarget, 6(31), 30975-30992.

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