Hep-3B cells were seeded into a 96-well plate with 3,000 cells per well. The 96-well plate was placed in a cell incubator. After the cells adhered to the wall, the corresponding irradiation dose(0Gy-10Gy) was given. After 5 days, the 96-well plate was taken out, the medium in the wells was aspirated, and then 100uL of the prepared CCK-8(CK04, DOJINDO, Japan) solution was added to the relevant wells (CCK-8: medium = 1:9). After that, the 96-well plate added with CCK-8 was placed in an incubator for 2 h, and then the absorbance at a wavelength of 450 nm was measured by an automatic microplate reader. Statistical data and cell proliferation curves were performed through Graphpad software.
Cck 8
Manufactured by Dojindo Laboratories
Sourced in Japan, United States, China, Germany
CCK-8 is a cell counting kit used to measure cell viability and proliferation. It utilizes a water-soluble tetrazolium salt that is reduced by living cells, producing a colored formazan dye that can be quantified using a spectrophotometer. The amount of formazan dye produced is directly proportional to the number of living cells in the sample.
Automatically generated - may contain errors
Lab products found in correlation
Microplate reader, by Bio-Rad (183 mentions)
Microplate reader, by Thermo Fisher Scientific (182 mentions)
Microplate reader, by Agilent Technologies (119 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (68 mentions)
Cck 8, by Agilent Technologies (59 mentions)
Cck 8, by Bio-Rad (55 mentions)
Microplate reader, by Molecular Devices (42 mentions)
Multiskan fc, by Thermo Fisher Scientific (42 mentions)
Cck 8, by Thermo Fisher Scientific (41 mentions)
Microplate reader, by Tecan (31 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (29 mentions)
96 well plate, by Corning (28 mentions)
Cck 8, by Molecular Devices (27 mentions)
Cell counting kit 8 (cck8), by Dojindo Laboratories (27 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (27 mentions)
Elx800, by Agilent Technologies (24 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (24 mentions)
Cck 8, by Tecan (23 mentions)
Elx808, by Agilent Technologies (21 mentions)
Synergy h1, by Agilent Technologies (20 mentions)
3 556 protocols using cck 8
1
Hep-3B Cell Proliferation Assay
2
IL-9 Modulates PANC-1 and AsPC-1 Proliferation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The PANC-1 and AsPC-1 cells were treated with different concentrations of IL-9 for 48 h. After that, cells were seeded at a density of 104 per well in 96-well plates and the media were changed to without IL-9. No vehicle control was used. Then, the Cell Counting Kit-8 (CCK-8) assay was used to detect cell proliferation according to the manufacturer's protocol; 1 × 104 cells were plated in 96-well microplates, and then 10 μl of CCK-8 (Dojindo, Beijing, China) solution was added to each well and the samples were incubated for 1 h prior to measuring absorbance at 450 nm. Experiments were performed in triplicate.
No vehicle control was used.
No vehicle control was used.
3
Cell Viability and Proliferation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cell suspension was evenly spread on a 96‐well plate with a volume of about 50 µL. According to different experimental requirements, another 50 µL containing drugs were added, with a total volume of 100 µL. Or 100 µL of stable lentivirus transfected cell suspension was added directly to the 96‐well plate. For CCK8, 10 µL of CCK8 (Dojindo, Japan) reagent was added. After 2 h in a constant temperature incubator at 37°C, the optical density value was measured by an enzyme marker at the wavelength of 450 nm, and the cell viability was evaluated by the difference of the optical density value. This method can also be used to detect cell proliferation. In the Neurosphere formation experiment, cell counts and images were taken under an inverted microscope for easy quantification. For the limiting dilution assay, medium with different drug concentration was configured first. After counting the total stem cell suspension, different cells were added to the prepared medium for blending, and then added to the 96‐well plate to reduce the error in the repeated experiment. Finally, the cell ball was counted. Detailed experimental methods can be seen in previous studies.32
4
Cell Viability Assay with NTS and MSM
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
THP-1 cells (1 × 104 cells/100 μL) treated with various concentrations of NTS or MSM were seeded into 96-well plates and incubated for 72 h. After 72 h, the cultured cells were harvested, and the cells with 100 µL of medium containing CCK-8 (Dojindo, Kumamoto, JAPAN; the medium and CCK-8 volume ratio was 9:1) without FBS was added to each well of new plate. After incubation for 2 h at 37 °C in a CO2 incubator, the absorption at 450 nm of each well was measured using a microplate reader (BioTek, Winooski, VT, USA).
5
Measuring Cellular Proliferation Rates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cellular proliferation rate was measured using CCK-8 (DOJINDO) as previously described[21 (link)]. To measure the effects on cellular proliferation rates, cells were incubated in 10% CCK-8 (DOJINDO) diluted in normal culture media at 37 °C until the appearance of visual color. Proliferation rates were determined at 12, 24, 36, 48, and 60 h post-transfection and quantification was done on a microtiter plate reader (Spectra Rainbow, Tecan) using the protocol recommended by the manufacturer.
6
Proliferation Assays for Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
EdU incorporation assay: To evaluate the proliferation of the distinct treated cells, 50 μM 5-ethynyl-2′-deoxyuridine (EdU; Ribobio, China) was added to the medium for 4 h. To determine the incorporation of EdU, the cells were fixed with 4% paraformaldehyde for 30 min at room temperature, and immunostained (red) using a standard protocol; in addition, Hoechst stain (blue) was used to visualize the nucleus. EdU incorporation was viewed and captured.
CCK 8 assay: Cells were incubated in 10% CCK-8 (Dojindo; Kumamoto, Japan) that was diluted in normal culture medium at 37° C until the visual color conversion occurred. Proliferation rates were determined at 0, 24, 48, and 72 hours after transfection.
CCK 8 assay: Cells were incubated in 10% CCK-8 (Dojindo; Kumamoto, Japan) that was diluted in normal culture medium at 37° C until the visual color conversion occurred. Proliferation rates were determined at 0, 24, 48, and 72 hours after transfection.
7
Evaluating Antiproliferative Activities of Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The antiproliferative activities
of the isolated compounds were evaluated against the A549 and P388
cell lines using cell counting kit-8 (CCK-8) assay.27 (link) Briefly, cells (10 × 103 cell/well) were
seeded in 96-well plates and grown for 24 h. Cells were then treated
with increasing concentrations of compounds and grown for further
72 h. At the end of exposure time, 10 μL of CCK-8 (Dojindo,
Kumamoto, Japan) was added to each well, and the plates were kept
in an incubator for 4 h and then measured at 450 nm using a multiwell
spectrophotometer (SpectraMax, Molecular Devices, U.S.A). The inhibition
rate was calculated using the following relation: (1 – A450 treated/A450 control) × 100%. The cytotoxicity of compounds was expressed
as IC50, determined by the Logit method. Adriamycin was
used as the positive control with IC50 values of 0.13 and
0.10 μM on A549 and P388 cancer cells, respectively.
of the isolated compounds were evaluated against the A549 and P388
cell lines using cell counting kit-8 (CCK-8) assay.27 (link) Briefly, cells (10 × 103 cell/well) were
seeded in 96-well plates and grown for 24 h. Cells were then treated
with increasing concentrations of compounds and grown for further
72 h. At the end of exposure time, 10 μL of CCK-8 (Dojindo,
Kumamoto, Japan) was added to each well, and the plates were kept
in an incubator for 4 h and then measured at 450 nm using a multiwell
spectrophotometer (SpectraMax, Molecular Devices, U.S.A). The inhibition
rate was calculated using the following relation: (1 – A450 treated/A450 control) × 100%. The cytotoxicity of compounds was expressed
as IC50, determined by the Logit method. Adriamycin was
used as the positive control with IC50 values of 0.13 and
0.10 μM on A549 and P388 cancer cells, respectively.
8
Cytotoxicity Assay of Drug-Resistant Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The growth rates of MGC803 and MGC803‐resistant cells treated with DMSO and 10058‐F4, respectively (four groups: MGC803/DMSO, MGC803/10058‐F4, MGC803‐resistant/DMSO, and MGC803‐resistant/10058‐F4), were detected by the CCK‐8 assay according to the manufacturer's protocol (CCK‐8; Dojindo Molecular Technologies Inc., Kumamoto, Japan). Briefly, 2000 cells per well were seeded into 96‐well tissue culture plates in a final 200 μL volume of growth medium. After 96 h incubation, absorbance at 450 nm was measured with an Enspire Multi‐label Reader 2300 (Perkin Elmer, Waltham, MA). Each experiment was performed in triplicate.
9
CCK-8 Assay for Cell Viability
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Once the cells reached 90% confluence (inverted microscope; Olympus Corporation), conventional hemocytometer-based cell counting was performed. Each of the 60 wells in the center of a 96-well plate was pipetted with 100 µl of cells (concentration 2×105 cells/ml), and the surrounding wells were pipetted with an equal volume of PBS (100 µl). Cells were labeled and incubated at 37°C for 24 h. After 24 h of serum deprivation in an equal volume of serum-free medium, the cells were washed once with PBS. Then cells were grouped (normal control group, connexin 43 group and small-interfering RNA group). Six replicates were performed for each of the groups, and the cells were cultured for 24 h after treatment. A 10% CCK-8 solution was prepared by adding 1.2 ml of CCK-8 (Dojindo Molecular Technologies, Inc.) into 12 ml of Endothelial Cell Medium (ScienCell Research Laboratories, Inc.). The treated cells were washed with PBS, and 100 µl of 10% CCK-8 was added to each well. Following incubation for 3 h, the absorbance (OD value) at 450 nm was measured using a microplate reader.
10
Cytotoxicity Assay of Anti-Cancer Drugs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were seeded in 96-well plates and treated with drugs (Erltinib,5μM; Geftinib,10μM; Cerubidine,1μM; Adriamycine,5μM; Idamycin,5μM; Ara-C,1μg/ml) once every day for 11 days. Cell viability was measured by the CCK-8 assay (CCK-8, Dojindo Laboratories, Kumamoto, Japan) according to the manufacturer’s instruction. All experiments were independently repeated at least 3 times. The survival rate was evaluated by cell counting kit-8 (CCK-8; Dojindo Molecular Technologies Inc., Gaithersburg, MD, USA). Cells were seeded in 48-well plates with RIPM1640 plus 10% FBS. The IC50 value was calculated using Statistical Package of the Social Sciences (SPSS) software version 12 (SPSS Inc., Chicago, IL, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!