The chemical compositions of the essential oils were analyzed using GC-MS. The analysis was performed on an Agilent Technologies Gas Chromatograph 7820A connected to an Agilent Technologies 5977B mass spectrometer system (Agilent, Santa Clara, CA, USA) based on electron impact (EI) and 70 eV of ionization energy. The gas chromatograph was equipped with a split/splitless injector and a capillary column HP5MS of 30 m, internal diameter of 0.25 mm and membrane thickness of 0.25 μm. The temperature program included an initial temperature of 60 °C for 5 min; then, an increase at a rate of 3 °C/min until the temperature reached 130 °C; then, an increase at a rate of 2 °C/min up to 180 °C; and finally, an increase at a rate of 5 °C/min with a final temperature of 240 °C, where the program was completed. The total analysis time was 65.33 min. The carrier gas was He at a flow rate of 0.7 mL/min, injection volume of 2 μL, split ratio of 1:10 and injector temperature of 280 °C. The compounds were identified via mass spectra comparison with libraries (WileyRegistry of Mass Spectral Data) and confirmed via a comparison of Kovats retention indices (KIs) with literature data [36 ].
7820a gas chromatograph
Manufactured by Agilent Technologies
Sourced in United States, Germany, France
The 7820A gas chromatograph is a laboratory instrument designed for the separation and analysis of various chemical compounds. It functions by using a carrier gas to transport the sample through a column, where the different components are separated based on their interactions with the column material. The 7820A gas chromatograph provides precise and reliable performance for a wide range of analytical applications.
Automatically generated - may contain errors
Lab products found in correlation
Hp 5 capillary column, by Agilent Technologies (4 mentions)
5977e mass spectrometer, by Agilent Technologies (3 mentions)
5975c network mass spectrometer, by Agilent Technologies (2 mentions)
Hp 5ms, by Agilent Technologies (2 mentions)
5975c network mass spectrometer gc ms, by Agilent Technologies (2 mentions)
Db 1ms, by Agilent Technologies (1 mentions)
7890a gas chromatograph, by Agilent Technologies (1 mentions)
Db 624 capillary column, by Agilent Technologies (1 mentions)
Hp 5 column, by Agilent Technologies (1 mentions)
Nicolet is10 ftir, by Thermo Fisher Scientific (1 mentions)
Hp 5ms column, by Agilent Technologies (1 mentions)
Thermo hp 5ms column, by Agilent Technologies (1 mentions)
Pufa2, by Merck Group (1 mentions)
Av 400, by Bruker (1 mentions)
Av 300, by Bruker (1 mentions)
Pe 5 capillary column, by PerkinElmer (1 mentions)
Masshunter software, by Agilent Technologies (1 mentions)
Db wax, by Agilent Technologies (1 mentions)
Gc fid, by Agilent Technologies (1 mentions)
Fid detector, by Agilent Technologies (1 mentions)
32 protocols using 7820a gas chromatograph
1
GC-MS Analysis of Essential Oil Composition
2
Monosaccharide Analysis using AMG Derivatives
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Monosaccharides were analyzed as acetylated methyl glycoside (AMG) derivatives as reported (Adinolfi et al., 1996 ). Briefly, 0.5–1 mg of sample was subjected to a methanolysis reaction (1.25 M HCl/MeOH, 1 ml, 80°C, 16 h). The obtained O-methyl-glycosides were extracted three times with hexane and the methanol layers were acetylated with Ac2O (50 μl) and Py (50 μl) at 100°C for 30 min and analyzed by using an Agilent Technologies gas chromatograph 7,820A equipped with a mass selective detector 5977B and an HP-5 capillary column (Agilent, 30 m × 0.25 mm i.d.; flow rate, 1 ml/min, He as carrier gas). The following temperature program was used for the analysis of the AMG: 140°C for 3 min, 150°C → 240°C at 3°C/min. The hexane layer containing fatty acid methyl esters (FAME) were analyzed by GC-MS to obtain fatty acid composition, by using the following temperature program: 140°C for 3 min, 140°C → 280°C at 10°C/min, and 280°C for 20 min.
3
GC-MS Analysis of Propolis Extract
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The component analysis was performed by the technique of gas chromatography coupled with mass spectrometry (Gas Chromatography–Mass Spectrometry, GC-MS). The analysis was performed on an Agilent Technologies Gas Chromatograph 7820A, connected to an Agilent Technologies 5977B mass spectrometer system based on electron impact (EI) and 70 eV of ionization energy. The gas chromatograph is equipped with a split/splitless injector and a capillary column HP5MS of 30 m, internal diameter of 0.25 mm, and membrane thickness of 0.25 μm. The temperature was programmed from 100 to 300 °C at a rate of 5 °C/min. The carrier gas was He at a flow rate of 0.7 mL/min, injection volume of 2 μL, split ratio of 1:10, and injector temperature of 280 °C. The identification was accomplished using computer searches on Wiley mass spectral databases (and database created from our research team). The components of propolis extract were determined by considering their areas as percentage of the total ion current.
4
Monosaccharide Analysis by GC-MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The monosaccharides were analyzed as acetylated methyl glycosides by GC-MS [28 (link)]. Briefly, an aliquot of LPS sample (0.5 mg) was subjected to a methanolysis reaction with HCl/CH3OH (1.25 M, 1 mL) at 80 °C for 16 h. The methanol was extracted three times with hexane to separate the fatty acid methyl esters from the O-methyl glycosides. The hexanic phase was dried and analyzed by GC-MS. The methanolic phase was dried, and then, the methyl glycosides were acetylated with acetic anhydride in pyridine at 100 °C for 30 min. The absolute configuration of the sugars was determined by subjecting the acetylated (S)-2-octyl glycosides to GC-MS [30 (link)]. The samples were analyzed on an Agilent Technologies gas chromatograph 7820A equipped with a mass selective detector 5977B and an HP-5 capillary column (Agilent, 30 m × 0.25 mm i.d.; flow rate, 1 mL min−1, He as carrier gas).
Acetylated methyl glycosides were analyzed using the following temperature program: 140 °C for 3 min, then 140→240 °C at 3 °C min−1. The temperature program for the analysis of acetylated octyl glycosides: 150 °C for 5 min, 150→240 °C at 6 °C min−1, 240 °C for 5 min.
Acetylated methyl glycosides were analyzed using the following temperature program: 140 °C for 3 min, then 140→240 °C at 3 °C min−1. The temperature program for the analysis of acetylated octyl glycosides: 150 °C for 5 min, 150→240 °C at 6 °C min−1, 240 °C for 5 min.
5
Monosaccharide and Fatty Acid Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Monosaccharides were analyzed as acetylated methyl glycosides, as reported previously [61 (link)]. Briefly, 1mg of sample was dried over P2O5 for 1 h; and the methanolysis was performed in 1 mL of HCl/MeOH 1 M at 80 °C for 20 h. The obtained product was extracted three times with hexane, and the methanol layer was dried and acetylated with 50 µL of Ac2O and 50 µL of Pyr at 100 °C for 30 min.
The hexane layer containing fatty acids methyl esters was also analyzed to obtain fatty acids composition. The samples were analyzed on an Agilent Technologies gas chromatograph 7820A equipped with a mass selective detector 5977B and an HP-5 capillary column (Agilent, 30 m × 0.25 mm i.d.; He as carrier gas). Acetylated methyl glycosides were analyzed using the following temperature program: 140 °C for 3 min, then 140 → 240 °C at 3 °C min −1. Finally, fatty acids methyl esters were analyzed with the following temperature program: 140 °C for 3 min, then 140 → 280 °C at 10 °C min −1, at 280 °C for 20 min.
The hexane layer containing fatty acids methyl esters was also analyzed to obtain fatty acids composition. The samples were analyzed on an Agilent Technologies gas chromatograph 7820A equipped with a mass selective detector 5977B and an HP-5 capillary column (Agilent, 30 m × 0.25 mm i.d.; He as carrier gas). Acetylated methyl glycosides were analyzed using the following temperature program: 140 °C for 3 min, then 140 → 240 °C at 3 °C min −1. Finally, fatty acids methyl esters were analyzed with the following temperature program: 140 °C for 3 min, then 140 → 280 °C at 10 °C min −1, at 280 °C for 20 min.
6
Acetylene Reduction Assay for Nitrogen Fixation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ARA were conducted on single plants using a protocol derived from [36] (link). Briefly, a single nodulated plant was placed into a 10 ml glass vial sealed with a rubber septum. 250 µl of acetylene were injected per vial. Plants were incubated for at least one hour at room temperature and gas samples (200 µl) were analyzed by gas chromatography using the 7820A Gas Chromatograph from Agilent Technologies (Santa Clara, USA) equipped with a flame ionization detector and a GS-Alumina column (50 m×0.53 mm) with hydrogen as carrier gas. Column temperature and gas flow rate were 120°C and 7.5 mL/min, respectively.
7
GC-MS Analysis of Drug Reference Standards
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
GC-MS analysis of reference standards (100–1,000 μg/mL) and seized sample extracts was carried out using a 7820A gas chromatograph coupled to a 5977E mass spectrometer (Agilent Technologies, Santa Clara, CA, USA). Injection mode: 1 μL sample injection and 20:1 split (and a 5:1 split for trace component screening), injection port temperature: 200°C, carrier gas: He, flow: 1 mL/min. Column: DB-1MS, 0.33 μm, 0.2 mm × 25 m (Agilent Technologies) (EMCDDA, 2017d ). GC oven: 80°C held for 3 min; 40°C/min to 300°C held for 3.5 min; transfer line: 295°C. The mass spectrometer operated in electron ionization (EI) mode. Ionization conditions: 70 eV in full scan mode (50–550 amu), ion source: 230°C, quadrupole: 150°C.
8
Gas Chromatography with FID Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Analyses were performed with a 7820A gas chromatograph (Agilent Technologies, Santa Clara, CA, USA) with a flame ionization detector (FID) as described previously (29 (link))
9
Biogas Production Protocol Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total solids, VS, COD, pH, alkalinity, and ammonia were analyzed according to Standard Methods [64 ] procedures 2540 B, 2540 E, 5250 D, 4500-H+B, 2320 B, and 4500-NH3D, respectively. Digester biogas was collected in Tedlar® bags and was measured daily by a U-Tube type manometer, while its composition was analyzed by an Agilent 7820A Gas Chromatograph equipped with a thermal conductivity detector [65 (link)]. Total VFAs (sum of acetic, propionic and butyric acids) were measured by an Agilent 7890A Gas Chromatograph with a flame ionization detector and Agilent 19091F-112 capillary column [66 (link)].
10
CO Conversion in CO2-rich Atmosphere
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Before the activity tests, all the catalysts were pretreated with H2 at 300 °C for 1 h. The CO conversion test was conducted in a CO2-rich atmosphere. The composition of raw gas was: 60 vol% CO2 + 1 vol% CO + 0.5 vol% O2, balanced with N2. The gas mixture passed through the U-shaped quartz reactor at a speed of 100 mL min−1. The reaction temperature was 220 °C. An Agilent 7820A gas chromatograph was used to determine the CO content. For the CO conversion test in CO2 free atmosphere, the composition of raw gas contained 1 vol% CO + 0.5 vol% O2, balanced with N2. The gas mixture passed through the U-shaped quartz reactor at a speed of 100 mL min−1. The reaction temperature was 220 °C.
The reaction rate was tested with a gas flow of 200 mL min−1, and the gas compositions and reaction temperature were exactly the same as those shown previously. A portion (0.01 g) of catalyst and 0.09 g of diluent (Al2O3) were loaded into the reaction tube. The CO content was determined by a gas chromatography.
The reaction rate was tested with a gas flow of 200 mL min−1, and the gas compositions and reaction temperature were exactly the same as those shown previously. A portion (0.01 g) of catalyst and 0.09 g of diluent (Al2O3) were loaded into the reaction tube. The CO content was determined by a gas chromatography.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!