Fluorescent and bright-field images were taken with a Zeiss Axiozoom.V16 epifluorescence microscope. Image acquisition and processing for whole-mount embryos were carried out using an AxioCam MRc Rev3 camera and the ZEN 2.0 software package. To prepare head cartilage sections, stage ~46 tadpoles (wild-type and snai2:eGFP) were fixed as described63 , embedded in Optimum Cutting Temperature media, and immediately frozen on dry ice. Embedded tadpoles were stored at −80 °C until being sectioned. Tadpoles were sectioned as previously described with a Leica CM3050 S cryostat at −35 °C64 . Air-dried sections were visualized using a Zeiss Axiozoom.V16 epifluorescence microscope.
Axio zoom v16
Manufactured by Zeiss
Sourced in Germany, France, United Kingdom, Denmark, Canada, Japan
The Axio Zoom V16 is a high-performance stereomicroscope from ZEISS. It offers a wide zoom range and high optical performance for various applications. The microscope provides a magnification range of 8x to 160x and a numerical aperture of 0.3, ensuring detailed observation and analysis of samples.
Automatically generated - may contain errors
Lab products found in correlation
Axiocam 506, by Zeiss (50 mentions)
Axio observer z1, by Zeiss (48 mentions)
Axiocam 503, by Zeiss (39 mentions)
Lsm 880, by Zeiss (36 mentions)
Axiocam mrc, by Zeiss (30 mentions)
Axiocam mrm, by Zeiss (24 mentions)
Zen software, by Zeiss (19 mentions)
Dfc310fx camera, by Zeiss (18 mentions)
Axiocam mrm camera, by Zeiss (10 mentions)
Tricaine, by Merck Group (10 mentions)
Zen pro software, by Zeiss (10 mentions)
Axio imager m2, by Zeiss (10 mentions)
Axiocam mrm monochrome camera, by Zeiss (10 mentions)
Zen 3.5 blue version software, by Zeiss (9 mentions)
Axiocam 512, by Zeiss (8 mentions)
Axiocam 105 color, by Zeiss (8 mentions)
Rhodamine 123, by Thermo Fisher Scientific (8 mentions)
Lsm 800, by Zeiss (8 mentions)
Zen pro, by Zeiss (8 mentions)
M165 fc, by Leica (8 mentions)
490 protocols using axio zoom v16
1
Tadpole Head Cartilage Imaging Protocol
2
Whole-mount Tissue Imaging Techniques
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Isolated organs and tissues were washed several times in PBS to clear blood, and the excess tissues were removed under the bright field of the microscope. Then placed on transparent agar in an anatomical direction to obtain the bright-field whole-mount images and fluorescent images by the Zeiss stereoscope (AxioZoom V16). We applied the automatic z-stack image function by the Zeiss stereoscope (AxioZoom V16) to obtain the magnification of a specific area.
3
Whole-mount Organ Imaging Protocol
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Collected visceral organs were washed in PBS and placed on agar in the required direction for whole-mount bright-field and fluorescence images by using a Zeiss stereoscope (AxioZoom V16). To determine the magnification of specific regions, we used the automated z-stack images acquired with a Zeiss stereoscope (AxioZoom V16).
4
Whole-Mount Imaging of Mouse Liver
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Collected mouse liver was washed in PBS and placed on agar for the whole-mount bright-field and fluorescence imaging using a Zeiss stereoscope (AxioZoom V16). To determine magnification of specific regions, we used the automated z-stack images acquired by the stereoscope (Zeiss AxioZoom V16).
5
Fluorescence Microscopy Imaging of NM-GFP-CM Fibers
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Phase-contrast images of the NM-GFP-CM fiber were observed by the fluorescence stereomicroscope (ZEISS Axio Zoom V16) equipped with a 7x, 25x, 50, and 100x lens. For the fluorescence images, the 7x lens required 550 msec exposure time, others were set as 280 msec. Tri-channel (EGFP, DAPI, DsRed) fluorescent images were captured at the same magnification (80x), with exposure time of 90 ms, using Hamamatsu ORCA-Flash4.0 V3 sCMOS camera equipped in ZEISS Axio Zoom V16 in 16 bit and processed using Zen Software (Zeiss, Stuttgart, Germany).
6
Live Imaging of Lymphatic Vessels
7
Live Imaging of Lymphatic Vessels
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Live imaging was performed on sedated Lyve1CreERTtdT mice. The mouse was positioned laterally and the globe exposed by retracting the eyelids to expose the limbus near the lateral canthus. Images were obtained using a Leica MZ10F Fluo III microscope using a Leica Planap 1.0× objective and a Leica DFC310FX camera (acquisition software: LAS version 4.0.0.8777) or a Ziess Axio Zoom.V16 and a Zeiss Plan-NeofluarZ 1.0 0.25 na objective and a Zeiss AxioCam MRm camera (acquisition software: Zeiss Zen 2012, blue edition, version 1.1.1.0).
8
Intravital Imaging of Mouse Pinna Lymphatics
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Live imaging was performed on sedated Lyve1CreERTtdT mice. The pinna was depilated and placed between glass slides, and the mouse was positioned laterally. Images were obtained using a Leica MZ10F Fluo III microscope using a Leica Planap 1.0X objective and a Leica DFC310FX camera (acquisition software: LAS version 4.0.0.8777) or a Ziess Axio Zoom.V16 and a Zeiss Plan-NeofluarZ 1.0 × 0.25 na objective and a Zeiss AxioCam MRm camera (acquisition software: Zeiss Zen 2012, blue edition, version 1.1.1.0). To acquire the clonal and small population LEC data, we used low magnification light microscopy to identify the major blood vessels within the pinna. These large stable structures were used to develop a vascular map of the pinna. Using this information to provide image guidance and the identical power of magnification, we were able to visualize the same fields of interest using light and fluorescent microscopy.
9
Quantifying Zebrafish Fluorescent Cells
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Zebrafish embryos were decorionated using 1 mg/ml Protease from Streptomyces griseus (Sigma). For Lightsheet imaging (Zeiss), embryos were screened for fluorescence using a fluorescent microscope (Zeiss axio zoom V16), and mounted in a column of 1% low melting point agarose (Sigma). Zebrafish embryos were imaged using dual side illumination, with maximum intensity projection (MIP) processed images generated using the Zen Black imaging software (Zeiss).
For assessment of the relative proportions of red and green fluorescent cell populations, 10 embryos from each embryonic stage were imaged on a fluorescent microscope (Zeiss axio zoom V16) and pixel count for each fluorescent marker extracted using the ImageJ software package (27 (link)).
For assessment of the relative proportions of red and green fluorescent cell populations, 10 embryos from each embryonic stage were imaged on a fluorescent microscope (Zeiss axio zoom V16) and pixel count for each fluorescent marker extracted using the ImageJ software package (27 (link)).
10
Immunostaining of Cardiac Tissues
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Immunostaining was performed as according to previous protocols. 32 (link) Briefly, hearts and other organs were collected and washed in cold PBS to remove excessive blood, and then hearts or other tissues were fixed in 4% polyformaldehyde at 4°C. After washing in PBS several times, hearts or other tissues with fluorescence reporters were observed and photographed using Zeiss stereomicroscope (Zeiss AXIO Zoom. V16). Hearts and other tissues were then dehydrated in 30% sucrose in PBS at 4°C until tissues were fully penetrated. Cryosections of tissues were then collected and blocked with PBS supplemented with 0.2% triton X-100 and 5% normal donkey serum for 30 minutes at room temperature, followed by primary antibody incubation at 4°C overnight. Detailed first antibody information including manufacturer, catalog number and dilution could be found in the online-only Data Supplement. After developing with secondary antibodies, images were acquired by Zeiss stereomicroscope (Zeiss AXIO Zoom. V16), Zeiss confocal laser scanning microscope (LSM 880), or Olympus confocal microscope (FV1000, FV1200). The image data were analyzed by ImageJ (National Institutes of Health) software.
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