The lung metastasis mouse model for breast cancer has been previously described[22 (link)–24 (link)]. For bioluminescent tracking, human TNBC MDA-MB-231 cells with stable luciferase expression were generated as earlier described. Five to six-week-old female NSG (NOD/SCID/IL2rγ−/−) mice were used in compliance with the Animal Care and Use Committee of Houston Methodist Research Institute guidelines. To induce lung metastasis, 3 × 105 luciferase-transduced human MDA-MB-231 cells (100 μl) were injected into the lateral tail vein of NSG mice. Within an hour of cell inoculation, cellular bioluminescent signals were visualized using the International Veterinary Information Service (IVIS) imaging system (PerkinElmer, MA, USA) to confirm successful settlement of the MDA-MB-231 luciferase-expressing cells in the lungs. Three days after cell inoculation, cellular bioluminescent signals were imaged using the IVIS imaging system (PerkinElmer, Waltham, MA, USA). Bioluminescent signals in the lung tissues of each mouse were quantified to facilitate mouse grouping. With a cutting threshold of 1 × 106 total flux (p/s) per mouse, 24 tumor-bearing mice were randomly divided into three groups (8 mice per group): control, NK-92-treated, and ApEn-NK-treated.
Ivis imaging system
Manufactured by PerkinElmer
Sourced in United States
The IVIS imaging system is a non-invasive, in vivo imaging platform that enables the visualization and quantification of bioluminescent and fluorescent signals in small animal models. It provides high-sensitivity detection and high-resolution imaging capabilities for a variety of applications, including disease research, drug discovery, and gene expression studies.
Automatically generated - may contain errors
Lab products found in correlation
Living image software, by PerkinElmer (31 mentions)
D luciferin, by GoldBio (13 mentions)
D luciferin, by PerkinElmer (7 mentions)
D luciferin, by Promega (6 mentions)
Living image 4, by PerkinElmer (5 mentions)
Luciferin, by Promega (3 mentions)
C57bl 6 mice, by Charles River Laboratories (3 mentions)
Nod cg prkdcscid il2rgtm1wjl szj nsg mice, by Jackson ImmunoResearch (3 mentions)
Balb c nude mice, by Charles River Laboratories (3 mentions)
Escalab 250xi, by Thermo Fisher Scientific (2 mentions)
Luciferin, by GoldBio (2 mentions)
Varioskan flash reader, by Thermo Fisher Scientific (2 mentions)
Pgl4.50 luc2 cmv hygro vector, by Promega (2 mentions)
Fugene hd transfection reagent, by Promega (2 mentions)
Female c57bl 6 mice, by Charles River Laboratories (2 mentions)
Nod cb17 prkdcscid ncrcrl nod scid mice, by Charles River Laboratories (2 mentions)
Vivoglo, by Promega (2 mentions)
Nano glo luciferase assay substrate, by Promega (2 mentions)
Platemate robotic, by Thermo Fisher Scientific (2 mentions)
Multidrop combi robotic dispenser, by Thermo Fisher Scientific (2 mentions)
204 protocols using ivis imaging system
1
Breast Cancer Lung Metastasis Model in NSG Mice
2
In Vivo Fluorescent Imaging of Tumor Targeting
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Through the tail vein, mice were intravenously injected with 100 µL (at 5 μg/mL) of targeted and untargeted Abx conjugated with peptides labeled with Flamma 675 near-infra red fluorescent dye (BioActs) at the N-terminus during synthesis. After a 6-h circulation, mice were anesthetized, and in vivo images were taken using the IVIS imaging system (PerkinElmer, Waltham, MA). After in vivo imaging, the mice were sacrificed, and the tumors and other organs were isolated and subjected to ex vivo imaging using the IVIS imaging system, and the total flux (photon/second) was measured using Living Image 4.5 software (PerkinElmer).
3
Hep-3B Tumor Xenograft and Metastasis Model
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All experiments were performed in compliance with all relevant ethical regulations and in accordance with an Institutional Animal Care and Use Committee-approved protocol (No. 18080B). NCG mice (GuangDong GemPharmatech Co., Ltd.) aged 4–6 weeks were inoculated subcutaneously with 2.5 × 106 tumour cells (Hep-3B-luc), and after the tumour grew to 50–100 mm3, Mock T, CD133 CAR-T and CD133 CAR-T and PD-1 s cells were intravenously injected into NCG mice with Hep-3B. For metastatic mouse models, the NCG mice were intravenously injected with 2.5 million Hep3B-luc cells. The metastasis was confirmed by bioluminescence imaging (BLI) and then treated with a tail veil injection of 5 million Mock T, CD133 CAR-T or CD133 CAR-T and PD-1 s cells. BLI of tumour growth was performed using a PerkinElmer IVIS imaging system with Living Image software. Finally, the tumour weight was evaluated. Mice were euthanized when the tumour volume reached 2000 mm3 or when they showed any sign of sickness. For establishment of an in situ xenograft tumour model, 2.5 million Hep3B-luc cells were directly injected into the liver of NCG mice. The tumour was confirmed by BLI, and 5 million Mock T, CD133 CAR-T or CD133 CAR-T and PD-1 s cells were injected into the tail vein. BLI of tumour growth was performed using a PerkinElmer IVIS imaging system with Living Image software.
4
Bioluminescent Imaging of Luciferase Variants
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The MDA-MB-231 cells were raised in a 6-well microplate (Nunc) until reach 70% of confluence. The cells were transiently transfected with pcDNA3.1(+) vector encoding GLuc, MLuc, NanoLuc, RLuc8.6-535, RLuc8.6-535SG, ALuc16, or ALuc23. The cells were further incubated in a CO2 incubator for 2 days. The cells were then subcultured into a 96-well black-frame optical-bottom microplate and incubated one more day in the CO2 incubator.
The culture media of the microplates are decanted and washed once with a phosphate-buffered saline (PBS). The wells in the microplate were randomly separated into two sections. The following live cell imaging was conducted through simultaneously injecting a series of CTZ analogues dissolved in 40 µL HEPES buffer into the wells in a section using a 12-channel micropipette. The consequent BL intensities were determined with an IVIS imaging system (PerkinElmer). On the other hand, the wells in the other section were lysed with a lysis buffer (Promega) for 20 min. 20 µL of the lysates were moved to a fresh 96-well black-frame optical-bottom microplate. The lysates in the microplate was simultaneously mixed with 40 µL of CTZ analogues dissolved in HEPES buffer using a 12-channel micropipette. The optical intensities were immediately determined with the IVIS imaging system (PerkinElmer).
The culture media of the microplates are decanted and washed once with a phosphate-buffered saline (PBS). The wells in the microplate were randomly separated into two sections. The following live cell imaging was conducted through simultaneously injecting a series of CTZ analogues dissolved in 40 µL HEPES buffer into the wells in a section using a 12-channel micropipette. The consequent BL intensities were determined with an IVIS imaging system (PerkinElmer). On the other hand, the wells in the other section were lysed with a lysis buffer (Promega) for 20 min. 20 µL of the lysates were moved to a fresh 96-well black-frame optical-bottom microplate. The lysates in the microplate was simultaneously mixed with 40 µL of CTZ analogues dissolved in HEPES buffer using a 12-channel micropipette. The optical intensities were immediately determined with the IVIS imaging system (PerkinElmer).
5
Light-triggered Intratumoral Retention of TRFC NPs
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Light-triggered structural transformation for intratumoral retention and accumulation of TRFC NPs was investigated by monitoring intratumoral fluorescence via IVIS imaging systems (PerkinElmer) in real time. The 4T1-tumor-bearing mouse model was built by subcutaneous injection of 1 × 107 4T1 cells into the hind limb of each female Balb/c mouse. When the tumor size reached approximately 100 mm3, the mice were injected with TRFC NPs (10 mg kg−1) intravenously before fluorescence imaging. For experiment groups, the tumors were irradiated with light (660 nm, 0.25 W cm−2, 3 min) at 2 h or 4 h after injection with TRFC NPs, and the mice were imaged at preset time points (0.5, 2, 4, 6, 12, 24, 48 h). The mice in control group were treated in the same way as the experiment groups except for the absence of light irradiation.
6
In Vivo Imaging of Tumor Uptake
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BALB/c nude mice were subcutaneously injected with 1 × 107 JEG-3 cells in the flank. When JEG-3 tumors in BALB/c mice were 200–300 mm3 in size, IVIS imaging systems (PerkinElmer, USA) were used to assess mice at specified time points following intravenous administration of IR780-labeled Lipo or IR780-labeled Cy-Lipo, respectively. The fluorescence images were collected at pre-treatment or suitable time points (0.5 h, 2 h, 6 h, 12 h, and 24 h) after treatment.
7
Multi-Technique Characterization of Nanoparticles
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Morphological observation
was performed
under transmission electron microscopy (TEM, JEOL-2100 Japan). Zeta
potential and hydrodynamic diameter were measured by dynamic light
scattering (DLS) on a Zetasizer ZEN3600 (Malvern). FTIR was collected
on a PerkinElmer spectrophotometer. UV–vis-NIR spectra were
collected by UV–vis-NIR spectrophotometry Lambda 35 (PerkinElmer).
XPS analysis was carried out on an ESCALAB 250XI (Thermo Fisher Scientific)
at Shiyanjia club. PXRD analysis was performed by Rigaku MiniFlex
600 X-ray diffractometer (Cu Kα, λ = 1.5418 Å). The in vivo imaging experiments were studied using IVIS imaging
systems (PerkinElmer). An 808 nm NIR laser (STL808T1–7.0 W)
was purchased from Beijing STONE Laser.
was performed
under transmission electron microscopy (TEM, JEOL-2100 Japan). Zeta
potential and hydrodynamic diameter were measured by dynamic light
scattering (DLS) on a Zetasizer ZEN3600 (Malvern). FTIR was collected
on a PerkinElmer spectrophotometer. UV–vis-NIR spectra were
collected by UV–vis-NIR spectrophotometry Lambda 35 (PerkinElmer).
XPS analysis was carried out on an ESCALAB 250XI (Thermo Fisher Scientific)
at Shiyanjia club. PXRD analysis was performed by Rigaku MiniFlex
600 X-ray diffractometer (Cu Kα, λ = 1.5418 Å). The in vivo imaging experiments were studied using IVIS imaging
systems (PerkinElmer). An 808 nm NIR laser (STL808T1–7.0 W)
was purchased from Beijing STONE Laser.
8
Xenograft and Orthotopic Tumor Models for Prostate Cancer
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Male athymic nude mice, 4–6 weeks old (Harlan Laboratories), were housed in the University of Colorado Center for Comparative Medicine. Subcutaneous xenografts were generated by injecting human VCaP cells in the flank of mice as described [11 (link)]. Approximately, 2 million cells were used for each injection. When tumors were palpable they were used for scans. Treatment was carried out with a single intraperitoneal injection of etomoxir (20 mg/kg) immediately after the basal PET scan. After 24 h the mice were subjected to a second PET scan. Mice were euthanized when no longer radioactive. For orthotopic xenografts, PC3-LUC cells (PC3 cells expressing the luciferase reporter) were injected in the right anterior lobe of prostates and monitored by bioluminescence with intraperitoneal injection of luciferin (IVIS imaging systems, Perkin Elmer). TRAMP transgenic mice were purchased from JAX® Mice at 6–8 weeks of age. All procedures were carried out under an approved University of Colorado Animal Care protocol.
9
In Vivo Metastasis Assay via Tail Vein Injection
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For tail vein injection-based in vivo metastasis assays, cells were injected into the lateral tail vein (1-3×106 cells in 100 μL of phosphate-buffered saline) of 6-week-old male nude mice or NCG mice (Model Animal Research Center, MARC) (n=6 mice per group). For bioluminescence imaging, mice were anesthetized and administered with 150 mg/kg D-luciferin via intraperitoneal injection; 10-15 min after injection, bioluminescence was detected using an IVIS imaging system (PerkinElmer) and analyzed using Living Image software (PerkinElmer). After 4-8 weeks, the mice were sacrificed, and lung tissues were obtained for further histological examination to detect cancer metastasis. All animal experiments were performed under a protocol (IACUC#623918) approved by the Peking University Shenzhen Graduate School Institutional Animal Care (China).
10
In vivo tumor bioluminescence imaging
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Luciferase-expressing tumor cells were injected s.c. on the flank of the mice. In vivo bioluminescence imaging was performed using an IVIS imaging system (PerkinElmer) by i.p. injection of luciferin (GoldBio). After anesthetization with 2% isofluorane and injection of 150 mg/kg luciferin, mice were photographed under brightfield, and images were overlaid with luminescence data gathered over the maximum exposure period without pixel saturation (0.5 to 60 s).
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