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Tnf pecy7

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The TNF-PECy7 is a fluorochrome-conjugated antibody that binds to the Tumor Necrosis Factor (TNF) protein. It can be used for the detection and analysis of TNF-expressing cells in flow cytometry applications.

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Lab products found in correlation

Intracellular cytokine staining kit, by BD (1 mentions) Cd19 bv510, by BioLegend (1 mentions) Cd45.2 pe dazzle, by BioLegend (1 mentions) Cd8 bv785, by BioLegend (1 mentions) Cd62l pe cy7, by BioLegend (1 mentions) Thy1.1 percp, by BioLegend (1 mentions) Streptavidin apc, by BioLegend (1 mentions) Cd44 apc cy7, by BioLegend (1 mentions) Cd4 pac blue, by BD (1 mentions) Cd45.1 bv605, by BioLegend (1 mentions) Countbright beads, by Thermo Fisher Scientific (1 mentions) Ifn γ alexa fluor 700, by BioLegend (1 mentions) Lsrii flow cytometer, by BD (1 mentions) Golgiplug, by BD (1 mentions)

2 protocols using tnf pecy7

1

TNF Release Assay in γδ T-Cells

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For TNF release assays γδ T-cells were cultured with tumour cells as previously described for cytotoxicity assay. Cells were cultured for 15 minutes before the addition of 10µM TAPI-0 (TNF-α protease inhibitor 0; Biotechne) and TNF-PECy7 (MAb11; Biolegend). Cells were cultured for 4 hours before being harvested and stained for flow cytometry.
Ridgley L.A., Caron J., Dalgleish A, & Bodman-Smith M. (2023). Releasing the restraints of Vγ9Vδ2 T-cells in cancer immunotherapy. Frontiers in Immunology, 13, 1065495.
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2

Multiparameter Flow Cytometry Assay

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Peripheral blood was processed using High Yield Lysis buffer (Thermofisher) and stained with biotinylated CD16/CD32 (2.4G2, BD Biosciences), biotinylated isotype control (IgG2b κ isotype, BD Biosciences), and CD4-PacBlue, CD8- BV785, CD19-BV510, CD44- APC-Cy7, CD62L- PE-Cy7,Thy1.1- PerCP, CD45.1- BV605, CD45.2- PE-Dazzle, and streptavidin-APC (all from Biolegend). For intracellular cytokine staining, splenocytes were ex vivo stimulated at 37⁰C with 30nm OVA257–264 (SIINFEKL) peptide and 10ug/mL GolgiPlug (BD Biosciences). After 4 hours, cells were processed and stained using an intracellular cytokine staining kit (BD Biosciences) with TNF- PE-Cy7 and IFNγ- Alexafluor700 (all from Biolegend). Flow cytometry samples were acquired on an LSR II flow cytometer (BD Biosciences) and data were analyzed using FlowJo (Tree Star, San Carlos, CA) and Prism (GraphPad Software). Absolute cell numbers were calculated using CountBright Beads (Life Technologies) according to manufacturer’s instructions.
Morris A.B., Pinelli D.F., Liu D., Wagener M, & Ford M.L. (2020). Memory T cell-mediated rejection is mitigated by FcγRIIB expression on CD8+ T cells. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 20(8), 2206-2215.
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