Transgenic RNAi fly lines bearing UAS-IR-TBPH #1 (#38379), UAS-IR-TBPH #2 (#38377), UAS-IR-Maleless (#19691), UAS-IR-nonA (#26442), UAS-IR-DmRH1 (#46933), UAS-IR-Caz (100291), UAS-IR-Hrb87F (#100732) and UAS-IR-Hrb98DE (#29523) were obtained from Vienna Drosophila RNAi Center (VDRC) and the Bloomington Drosophila Stock Center at Indiana University (BDSC). The level of knockdown of TBPH in the #38379 line was reported to be an approximately 23% reduction compared with that in wild-type flies (Lin et al., 2011 (link)). Transgenic fly lines bearing UAS-EGFP (#6658, #6874), elav-GAL4 (#458) and UAS-IR-GFP (#9330) were obtained from BDSC. The transgenic fly line bearing GMR-GAL4 has been described previously (Yamaguchi et al., 1999 (link)). The genotypes of the fly lines used are described in Supplemental Table 3 . All experiments were performed at 25 °C unless otherwise stated. Eye phenotypes of 1–2-day-old flies were evaluated with the stereoscopic microscope model SZX10 (Olympus) and a scanning electron microscope (Miniscope TM1000; Hitachi High-Technologies Corporation.).
The fly lines were allocated to experimental groups based on genotype, equivalent age and gender. Where multiple groups of the same genotype were required, these were allocated randomly to the particular experimental conditions. Sample sizes were estimated using pilot experiments.
The fly lines were allocated to experimental groups based on genotype, equivalent age and gender. Where multiple groups of the same genotype were required, these were allocated randomly to the particular experimental conditions. Sample sizes were estimated using pilot experiments.