Cells were seeded at a density of 2 × 104 cells/well in 96‐well plates. After the cell confluence reached 80%, the cells were treated with 5‐HT from 10−9 to 10−3 M for 1 day and 3 days, respectively. The viability of cells were determined by the (3‐(4,5‐dimethylthiazol‐2‐yl‐)‐5‐(3‐carbxymethoxyphenyl)‐2‐(4‐sulfophenyl)‐2H‐tet‐razolium, innersalt) (MTS) assay. After medium removal, the cells were washed with PBS once, then 100 μl of MTS solution (Promega, China) consisting of 80 μl of MEMα and 20 μl of MTS solution were added to each well, and the cells were incubated for 3 h at 37°C. The absorption value (OD value) was measured at 490 nm using Multiskan Spectrum reader (Thermo Fisher). The results are expressed as the relative ratio to the control.
Mts solution
Manufactured by Promega
Sourced in United States, China, Italy
The MTS solution is a laboratory reagent used to measure cell viability and cytotoxicity in cell-based assays. It provides a colorimetric method for quantifying the number of viable cells present in a sample.
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Lab products found in correlation
Microplate reader, by Agilent Technologies (6 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (6 mentions)
Microplate spectrophotometer, by Agilent Technologies (5 mentions)
Microplate reader, by Molecular Devices (4 mentions)
Microplate reader, by Bio-Rad (4 mentions)
L glutamine, by Thermo Fisher Scientific (4 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Infinite 200 pro, by Tecan (3 mentions)
Multiscan go plate reader, by Thermo Fisher Scientific (3 mentions)
96 well plate, by Corning (3 mentions)
Varioskan flash, by Thermo Fisher Scientific (2 mentions)
Spectramax m5, by Molecular Devices (2 mentions)
96 multiwell culture plate, by BD (2 mentions)
Chemiluminescence detection kit, by Cytiva (2 mentions)
γ 32p atp, by Cytiva (2 mentions)
Mmp 1 antibody, by Thermo Fisher Scientific (2 mentions)
Protein assay kit, by Bio-Rad (2 mentions)
Versamax microplate reader, by Molecular Devices (2 mentions)
Tryple, by Thermo Fisher Scientific (2 mentions)
158 protocols using mts solution
1
5-HT Cytotoxicity Evaluation in Cell Lines
2
Cell Viability Assays in 2D and 3D
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The 2D cell viability assays were performed using KPC CAFs and CCs seeded at (250 CAFs per well) and (500 CCs per well) in a 96-well plate. MTS solution (Promega) was added to the cells at a 1:20 dilution and cells were incubated at 37 °C for 2 h. Cell proliferation rates were measured by detecting the absorbance at 490 nm using a microplate reader. Three biological repeats were performed.
The 3D cell viability assays were performed using cells embedded into polymerized collagen at 2.5 mg ml−1. KPC CAFs and CCs were seeded at 10,000 cells per well in a 96-well plate. MTS solution (Promega) was added to the cells at a 1:20 dilution and cells were incubated at 37 °C for 1.5 h. Cell proliferation rates were measured by detecting the absorbance at 490 nm using a microplate reader and three biological repeats were performed.
The 3D cell viability assays were performed using cells embedded into polymerized collagen at 2.5 mg ml−1. KPC CAFs and CCs were seeded at 10,000 cells per well in a 96-well plate. MTS solution (Promega) was added to the cells at a 1:20 dilution and cells were incubated at 37 °C for 1.5 h. Cell proliferation rates were measured by detecting the absorbance at 490 nm using a microplate reader and three biological repeats were performed.
3
MTS Cell Viability Assay
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Cells were seeded in 96-well plates at 0.5 × 105 for growth or 1 × 105 for drug sensitivity in each well. 20 μl MTS solutions (Promega, Madison, WI) were added to each well, and the cells were incubated for 4 h at 37 °C. The plates were read at a wavelength of 490 nm using Varioskan Flash (Thermofisher, USA).
4
Cell Viability Assay with MTS
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Cells were seeded in 96‐well plates at 0.5 × 105 for cell growth assay or 1 × 105 for drug sensitivity experiment each well. 20 μl MTS solutions (Promega, Madison, WI) were added to each well, and the cells were incubated for 4 h at 37°C. The plates were read at a wavelength of 490 nm using Varioskan Flash (ThermoFisher, USA).
5
Acetylshikonin and 3-DSC Cytotoxicity Assay
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Cells (5 × 103 cells/well) were seeded in 96-well plates and treated with different concentrations (5 µM, 10 µM, 20 µM, 40 µM, 80 µM) of acetylshikonin or 3-DSC. Cell proliferation was detected at various times (24, 48, and 72 h). For each well, 20 µl of the MTS solution (Promega, Madison, WI) was added. After 1 h of incubation, 25 µl of 10% SDS solution was added, and the absorbance was detected at 492 and 690 nm with a microplate spectrophotometer [22 (link)].
6
Cell Proliferation Assay Protocol
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A total of 100 μl cell suspension and 20 μl MTS solution (Promega G3580, China) were seeded in per well for 96-well plates. After 2 h, per well was tested at 490 nm. The proliferation rates of cells with different treatments were investigated at 0, 24, 48, and 72 h. The experiment was repeated three times.
7
Cell Proliferation Assay in 96-well Plates
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Cells were seeded into 96-well plates at a concentration of 200 cells per well and incubated for the indicated time points. Then 20 μL of MTS solution (Promega) was added to each well, followed by incubation at 37°C for 1 h. The absorbance at 490 nm was quantitatively measured in a microplate reader (BioTek, Winooski, VT, USA).
8
MTS Assay for Cell Viability
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To perform the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay, the medium was removed, and the cells were washed with PBS solution. Then a fresh medium containing 10% of MTS solution (Promega, WI, USA) was added to the samples and they were incubated at 37 °C for 1.5 h. After the incubation period, the medium was transferred to a 96-well plate (Sarstedt, Nümbrecht, Germany) and the absorbance was recorded at 490 nm using Polarstar Omega microplate reader (BMG Labtech, Ortenberg, Germany).
9
Cytotoxicity Assay for GBM Cells
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GBM cells (1 × 105/well) maintained in complete medium were placed in 96-well plates overnight. Salinomycin, celastrol, and triptolide at various concentrations or identical volume of control (DMSO) were added to the appropriate wells. The cells were treated for 48 h before adding MTS solution (Promega) according to the manufacturer's instructions. After 2–4 h incubation, the number of cells in each well was determined by measuring the optical densities at 490 nm. The results were expressed as the percentages of viable cells against the control cultures.
10
Cytotoxicity Evaluation of FD in BV2 Cells
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MTS solution (Promega, USA) was used to determine whether the FD concentrations used in the experiment caused any cytotoxicity in BV2 cells. This assay is based on the mitochondrial mediated reduction of a tetrazolium compound (MTS) by living cells to form a colored formazan product which is measured colorimetrically [15 (link), 16 (link)]. Briefly, cells were seeded on a 96-well plate at the density of 5 × 104 cells/well. After 24 h, the cells were incubated with FD (0, 1, 2, 4, and 8 mg/mL) for 24 h or 48 h. 20 μL of MTS/PMS solution (Promega, USA) was added to each well. After 3 h incubation at 37°C in 5% CO2, the concentration of the MTS formazan product was measured at 490 nm (Dynex MRX II microplate reader). The average of absorbance values for triplicate wells was examined. The absorbance values of all wells were deducted with the values of DMED-treated control, which served as the background reading, and reported in percentage (%) as cell viability.
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