Bacterial growth media including brain heart infusion agar, Mac Conkey agar, eosin methyl blue agar, sugarsbroth, triple sugar iron agar, urea broth, methyl red media, Voges-Proskauermedia, and Mueller Hinton agar (MHA) were obtained from Oxoid (Hampshire, UK). Paper discs containing standard antibiotics namely ampicillin 10 µg, amoxycillin 25 µg, chloramphenicol 30 µg, cefuroxyme 30 µg, cefotaxime 30 µg, cefoperazone 75 µg, cefepime 30 µg, meropenem 10 µg, amikacin 30 µg, tetracycline 30 µg, ciprofloxacin 5 µg, levofloxacin 5 µg, co-trimoxazole 25 µg, and ceftazidime 30 µg were purchased from Oxoid (Hampshire, UK). Reagents (chrystal violet, 96% ethanol, iodin, safranin O, ammonium oxalate, oksalat, para-dimethylaminobenzaldehyde, butanol, acid chloride, α-naphtol 5%, KOH 40%, and distilled water) were supplied by Microbiology Laboratory, Faculty of Medicine, Universitas Sumatera Utara (Medan, Indonesia).
Eosin methyl blue agar
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
Eosin methyl blue agar is a microbiological culture medium used for the isolation and differentiation of Gram-negative bacteria, particularly members of the Enterobacteriaceae family. It contains eosin and methylene blue as indicators, which assist in the identification of bacterial colonies based on their lactose fermentation and ability to produce acid. This agar provides a selective and differential environment for the growth of target microorganisms.
Automatically generated - may contain errors
Lab products found in correlation
Tetracycline, by Thermo Fisher Scientific (1 mentions)
Ciprofloxacin, by Thermo Fisher Scientific (1 mentions)
Brain heart infusion agar, by Thermo Fisher Scientific (1 mentions)
Triple sugar iron agar, by Thermo Fisher Scientific (1 mentions)
Urea broth, by Thermo Fisher Scientific (1 mentions)
Ampicillin, by Thermo Fisher Scientific (1 mentions)
Amoxycillin, by Thermo Fisher Scientific (1 mentions)
Chloramphenicol, by Thermo Fisher Scientific (1 mentions)
Cefotaxime, by Thermo Fisher Scientific (1 mentions)
Cefoperazone, by Thermo Fisher Scientific (1 mentions)
Cefepime, by Thermo Fisher Scientific (1 mentions)
Meropenem, by Thermo Fisher Scientific (1 mentions)
Amikacin, by Thermo Fisher Scientific (1 mentions)
Levofloxacin, by Thermo Fisher Scientific (1 mentions)
Cotrimoxazole, by Thermo Fisher Scientific (1 mentions)
Ceftazidime, by Thermo Fisher Scientific (1 mentions)
Macconkey agar, by Thermo Fisher Scientific (1 mentions)
Mueller hinton agar (mha), by Thermo Fisher Scientific (1 mentions)
Tryptone bile x glucuronide agar, by Thermo Fisher Scientific (1 mentions)
Api 20e, by bioMérieux (1 mentions)
3 protocols using eosin methyl blue agar
1
Bacterial Growth Media and Antibiotic Susceptibility Testing
2
Bacterial Isolation and Characterization
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Bacteria were isolated on three types of agars (Oxoid): eosin methyl blue agar, mannitol salt agar, and sheep blood agar. Clinically isolated culture stocks were plated onto each type of agar, and distinct colonies were assessed by gram staining and light microscopy. Distinction criteria were based on colony form, margin, elevation, differing hemolysis, mannitol fermentation, and type of agar. Isolated colonies were incubated in liquid growth medium overnight, then mixed with glycerol (150 μL/mL), and stored at −80°C until required.
3
Enumerating Thermo-tolerant Gram-Negative Bacilli
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Thermo-tolerant Gram-negative bacilli were enumerated on Tryptone Bile X Glucuronide agar (Oxoid CM0595, England). Thus, 1 ml of the 10 -1 to 10 -3 dilutions was poured into petri dishes and 15 ml TBX was added, mixed and homogenized before incubation at 44°C for 24 h. The search for gram-negative bacteria was completed, after isolation on Eosin Methyl Blue Agar (Oxoid CM0069, England), by the indole test and the Api20E (bioMérieux, France) gallery (Riegel et al., 2006) .
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