M1000 pro plate reader
The Tecan M1000 Pro is a plate reader designed for high-performance absorbance, fluorescence, and luminescence detection. It features a wide wavelength range and high-precision optics to enable accurate measurements across a variety of assays and sample types.
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35 protocols using m1000 pro plate reader
Curcumin-Loaded Polymer Characterization
Quantification of Protochlorophyllides in Arabidopsis
SARS-CoV-2 Spike RBD IgG Antibody ELISA
Evaluating CrCl3 Cytotoxicity with MTT Assay
using the MTT cell proliferation
assay.68 (link),69 (link) Briefly, 2 × 104 cells per
well were seeded onto Corning Scientific Costar 96-well polystyrene
flat bottom plates. After 24 h, the growth medium was aspirated and
replaced with new growth medium containing CrCl3. Sodium
dodecyl sulfate (0.05, 0.10, 0.15, or 0.20 mg/mL) was used as a positive
control for the assay.
After incubation for 1 h, the growth
medium was carefully aspirated and replaced with 100 μL of fresh
growth medium (absent of phenol red) and 10 μL of the MTT reagent.
After 6 h, the MTT solution was carefully aspirated and the formazan
crystals were dissolved with 50 μL of spectroscopic grade DMSO.
The absorbance at 540 nm was read by an M1000 PRO plate reader (Tecan,
Switzerland) following 1 s of shaking at 2 mm amp and 654 rpm. To
remove the background media effects, empty (blank) wells were also
treated according to the MTT protocol.
Cell Proliferation Assay via GFP Fluorescence
3D Culture and Drug Response Assay for TNBC
Quantifying Cardiac Creatine Kinase Activity
Spectrophotometric Assay for TPI Activity
Hemin-Catalyzed Oxidation of ABTS
Membrane Interactions of P0ct Protein
SAXD experiments were performed to investigate any repetitive structures in turbid samples. 10 and 20 µM P0ct was mixed with SUVs of 1–3 mM DMPC:DMPG (1:1) in HBS at ambient temperature and exposed at 25 °C on the EMBL P12 BioSAXS beamline, DESY (Hamburg, Germany). A HBS buffer reference was subtracted from the data. Lipid samples without added P0ct were devoid of Bragg peaks. The peak positions of momentum transfer, s, in P0ct-lipid samples were used to calculate mean real-space repeat distances, d, in proteolipid structures, using the equation
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